Effect Of Cancer Stem-like Stem Cell Exosomes On Proliferation And Invasion In Human Umbilical Cord Mesenchymal Stem Cells

Objective:To investigate whether Piwil2-iCSCs derived exosomes could have an effect on the proliferation,migration and invasion of human umbilical cord mesenchymal stem cell(hucMSCs).Methods: Piwil2-iCSCs derived exosomes were isolated by ultracentrifugation,then identified by transmission electron microscopy,Nanoparticle Tracking Analysis and western-blot.Exosome uptake assay was used to detect the pathway how the Piwil2-iCSCs derived exosomes works.HucMSCs were divided into control group(Control),PBS intervention group(PBS)and exosome intervention group(Exo).CCK-8,Wound healing assay,Transwell assay,western-blot and cell karyotype analysis were used to detecting the proliferation,migration,invasion,the expression levels of MMP2 and MMP9 proteins,and chromosome structure of hucMSCs.Results: The diameter of Piwil2-iCSCs derived exosomes was about 50nm~100nm,most of them were oval or spherical and coated with membrane and rich in CD9,CD63 and Piwil2 proteins.Exosomal uptake assay indicated that exosomes entered the cell to work.Compared with the Control group and the PBS group,the number of cell proliferation in the Exo group was significantly increased(P<0.05);the healing rate of scratches was accelerated(24h,P<0.05;48h,P<0.01);the number of invasive cells was significantly increased(P<0.01);the expression of MMP2(P<0.05 vs PBS group,P<0.01 vs Control group)and MMP9(P<0.05)protein levels in hucMSCs cells treated with Piwil2-iCSC exosomes increased;but the karyotype was still 46 XY with no abnormalities.Conclusion: Piwil2-iCSCs derived exosomes could promote the proliferation,migration and invasion of hucMSCs,and no cancer-like heterogeneity changes.