Suppressive Effect Of Human Umbilical Cord Mesenchymal Stem Cells Lysate On Proliferation And Migration Of Esophageal Cancer EC9706Cells In Vitro

Background Esophageal cancer is one of common gastrointestinal malignancy in our country and the world, It is a serious threat to human health, the comprehensive treatment based on surgery had despited a certain extent efficacy, but the5years survival rate is only8%to30%,the10years survival rate is only5.2%to24.0%. This requires us to develop new treatments for esophageal cancer therapy as soon as possible.Mesenchymal stem cells have the potential for being used in the clinical treatment of many diseases. The effect of MSCs on tumor cells remains to be further studied. Clarifying the relationship between MSCs and esophageal cancer is significant for people in-depth understanding of the characteristics of MSCs and clinical treatmentObjective The experiment is to investigate the suppressive effect of human umbilical cord mesenchymal stem cells (hUCMSCs) lysate on proliferation and migration of esophageal cancer EC9706cells in vitro. Finally I hope that this experiment will provide experimental evidence for stem cell therapy of esophageal cancer and explore new integrated approach for clinical treatment of esophageal cancer.Methods hUCMSCs was cultured by using tissue culture in vitro; cell phenotype analysis was performed by using flow cytometry. hUCMSCs was collected and hUCMSCs lysate was prepared through repeated freezing and thawing. Added different concentration of lysate to esophageal cancer EC9706cells and cultured for a period of time, cell proliferation was evaluated with MTT assay and cell morphology was observed by using inverted microscope. The flow cytometry is used to detect cell cycle and apoptosis after Lysates acting on EC9706cells a certain time. Tranwell experiment is used to observe the effect of hUCMSCs lysate on the migration of EC9706cell. Results When hUCMSCs was cultured for7-12days, adherent and fibroblast-like cells were visible. Flow cytometry analysis revealed that CD29、CD90and CD166were highly expressed on the plasma membrane of the5th generation cells, but negative for CD34.. CD45and HLA-DR.MTT assay showed that lysate obviously inhibited the growth of esophageal cancer cells compared with the control group when the adding number of hUCMSCs is equal to or greater than the number of esophageal cancer cell(P<0.05), and the inhibition of cell proliferation of each experimental group in esophageal cancer was no significant difference(P>0.05).The number of cancer cells of the experimental group was significantly less than the control group, a lot of cancers’cell membrane presents changes in insect bite, a lot of cells have deformed, like vacuoles, and there are no significant necrosis and floating cancer cells within the field of vision.Compared with the control group, the number of esophageal cancer cells in G2-M phase (P<0.05) and the number of apoptotic cells (P<0.01) have increased in the experimental group.Tranwell experiment showed that the experimental group EC9706cell migration was significantly inhibited compared with the control group (P<0.05).Conclusion HUCMSCs lysate has an inhibition effect on both proliferation and migration of esophageal cancer EC9706cells in vitro; HUCMSCs lysate inhibit the growth of esophageal cancer EC9706cells by regulating cell cycle and blocking tumor cells temporarily in the G2/M phase of the cell cycle, and inducing cell apoptosis.