Expression Of NCAPH,Axl And YAP In Laryngeal Squamous Cell Carcinoma And Its Relationship With Prognosis

Objective:By observing the expression of Non-SMC Condensin?Complex Subunit H(NCAPH),receptor tyrosine kinase Axl and Hippo signaling pathway transcription coactivator Yes-Associated Proton(YAP)in laryngeal carcinoma tissues,and analyzing of the survival of patients with laryngeal cancer included in the study,to explore the significance of NCAPH,Axl and YAP in the development and progression of laryngeal carcinoma and its guiding value for prognosis of laryngeal cancer.Methods:The data was collected in the 1st Affiliated Hospital of He'nan University of Science and Technology,China,from October 2012 to June 2013.40 cases of postoperative tissue wax specimens of laryngeal cancer patients were used as experimental group,and 40 cases of postoperative tissue wax specimens of vocal cord polyps were used as control group.Immunohistochemistry was used to detecting the expression of NCAPH,Axl and YAP in laryngeal carcinoma tissues and vocal cord polyps.Statistical methods were used to analyze the correlation between expressions and the relationship between expression of NCAPH,Axl and YAP and clinicpathological features of laryngeal squamous cell carcinoma.And completed 60-month retrospective follow-up from the surgery day,analysis of postoperative survival status of patients with laryngeal cancer.Using Cox proportional hazard model to analyze the effect of NCAPH,Axl and YAP expression in laryngeal carcinoma on postoperative overall survival time in patients with laryngeal cancer.Results:1.The high expression rates of NCAPH,Axl and YAP in laryngeal carcinoma tissues were higher than those in vocal cord polyps,the difference was statistically significant(P <0.05).2.There was a correlation between the expression of Axl and YAP in laryngeal carcinoma(P<0.05).There was no significant difference in the correlation of the expression between NCAPH with Axl and NCAPH with YAP in laryngeal carcinoma(P>0.05).3.The expression of NCAPH,Axl and YAP in laryngeal carcinoma were correlated with clinical stage,lymph node metastasis and tissue differentiation of laryngeal carcinoma,the difference was statistically significant(P<0.05).The clinicalstage is relatively higher,or with lymph node metastasis,or the degree of differentiation is relatively lower,the higher the high expression rate of NCAPH,Axl and YAP.There was no significant difference in age and anatomical division(P>0.05).4.Expression of NCAPH and YAP in laryngeal carcinoma is associated with postoperative survival in patients with laryngeal cancer,high expression of NCAPH and YAP suggests poor clinical outcomes,the difference was statistically significant(P<0.05).There was no significant correlation between the expression of Axl in laryngeal carcinoma and the postoperative survival time of patients with laryngeal cancer(P>0.05).Conclusions:1.NCAPH,Axl and YAP may be involved in the development,progression,invasion and metastasis of laryngeal carcinoma,and have important guiding significance in the prognosis of laryngeal squamous cell carcinoma.Combined detection of NCAPH,Axl and YAP is expected to be an effective indicator of a prognostic assessment of laryngeal cancer.2.There was a positive correlation between the expression of Axl and YAP in laryngeal carcinoma,suggesting that Axl and YAP may have positive regulatory effects in laryngeal carcinoma.