The Study On The Mechanism Of MAT2B Gene In Osteosarcoma

BACKGROUDOsteosarcoma is a common malignant tumor in adolescents.Most of them usually occur in limbs and othes are rare in other parts of the body such as maxillofacial region,ribs and so on.After the introduction of the chemoptherapy,the 5-year survival after surgery increased to 70%.However,the prognosis of patients with metastasis or recurrence is poor.The protein encoded by the MAT2 B gene is belong to the MAT(methionine aminotransferase)family.MAT catalyzes the synthesis of S-adenosylmethionine by methionine and ATP.The MAT2B-encoded protein is a regulatory subunit of MAT.MAT2 B is associated with many tumors,so we study the role of MAT2 B gene in the development of osteosarcoma to discover a new target for the treatment of osteosarcoma.OBJECTIVEThe study on the mechanism of MAT2 B gene in osteosarcomaMETHODSThe expression was detected by immunohistochemistry(osteosarcoma and normal bone tissue).The expression of the gene in osteosarcoma cells was detedcted by quantatitive real-time PCR.The lentivirus was constructed to knock down the gene expression.The cell Cytological experiments(MTT,apoptotic caspase3/7,Annexin-V-APC flow cytometry)were performed.The core genes were detected by microarray detection.The tumor model was established in nude mice.The growth curve was drawn after recording the tumor growth and parallel GFP imaging was conducted.RESULTSImmunohistochemistry showed that the expression of gene in osteosarcoma and normal bone tissue were significantly different.The expression of the gene in four kinds of osteosarcoma cells was high.Knockdown of the gene inhibited cell proliferation,promoted apoptosis and inhibited the growth of tumor.After studying the interaction between genes,we found that the inhibition of the growth of osteosarcoma cells was related to the regulation of the expression of CITED2,EGFR,PCNA and TNFRSF9.CONCLUSIONMAT2B in osteosarcoma tissue is upregulated,while interfering with MAT2 B both in vitro and in vivo can inhibit osteosarcoma growth and promote apoptosis.MAT2 B may become a new target for the treatment of osteosarcoma.