Expression Of MiR-34a-5p And Its Target Gene MMP2 In Peripheral Blood Of Patients With Acute Respiratory Distress Syndrome And Its Clinical Significance

Objective:The differential gene expression of miRNAs and their target genes were predicted by bioinformatics methods.To investigate the expression and clinical significance of related miRNAs and their target genes in peripheral blood of patients with ARDS,and to evaluate their significance in the diagnosis and prognosis of patients with ARDS.Methods:By using R language data analysis,GO and KEGG for gene function and pathway enrichment analysis methods to analyze and screen out differentially expressed miRNAs related to ARDS.Real-time quantitative polymerase chain reaction?qRT-PCR?was used to detect the expression levels of candidate miRNAs in peripheral blood of 80 patients with ARDS.According to the oxygenation index?PaO₂/FiO₂?,patients were divided into mild ARDS?200mmHg<PaO₂/FiO₂?300mmHg,n=20?,moderate ARDS?100mmHg<PaO₂/FiO₂?200mmHg,n=20?,severe ARDS?PaO₂/FiO₂?100mmHg,n=40?and 80 healthy subjects.The levels of serum MMP2 in 80 patients with ARDS and 80 healthy subjects were detected by enzyme-linked immunosorbent assay?ELISA?,and the clinical significance of the serum MMP2 was analyzed by statistical analysis.The correlations of candidate miRNA expression,serum MMP2,and APACHEII scores were known by correlation analysis.Afterward,ARDS patients were assigned to survivors and non-survivors,and a backward stepwise logistic regression model was built to screen independent risk factors for poor outcome in patients with ARDS.Results:?1?By mining data and analyzing and screening by bioinformatics methods,three miRNA related to ARDS were obtained:miR-155-5p,miR-1-3p,miR-34a-5p.?2?Screening for miR-34a-5p by module interaction analysis can target MMP2,and MMP2 is used for further clinical research.?3?The results of qRT-PCR indicated that miR-155-5p and miR-1-3p were highly expressed in peripheral blood of patients with ARDS,and showed low expression in healthy subjects,but statistically calculated in peripheral blood and health of patients with ARDS.There was no significant difference in the expression of the tester?P>0.05?;miR-34a-5p was significantly different from the healthy subjects in the peripheral blood of patients with ARDS,and with the severity of ARDS disease gradually increased,miR-34a-5p the expression gradually increased,and the comparison between the two groups showed statistical significance?P<0.05?.?4?Elisa was used to detect serum MMP2expression levels in four groups of subjects.The expression of serum MMP2 in patients with different severity was higher than that in healthy controls.The expression of serum MMP2 in patients with severe ARDS was higher than moderate and mild.Serum MMP2 expression was significantly higher in patients with ARDS than in mild patients?P<0.05?.?5?The expression of miR-34a-5p in peripheral blood of ARDS patients was positively correlated with the expression of MMP2?r=0.946,p<0.01?.?6?MiR-34a-5p and MMP2 were positively correlated with APACHEII scores?r=0.923,0.975,respectively,p<0.05?.?7?Logistic regression analysis showed that APACHE II score and miR-34a-5p elevation were independent risk factors for poor prognosis.Conclusions:?1?Through bioinformatics methods,we screened miR-155-5p,miR-1-3p and miR-34a-5p as candidate markers for ARDS patients;and through module interaction we selected MMP2 as candidate markers for ARDS patients.?2?Peripheral blood miR-34a-5p was highly expressed in patients with ARDS.?3?Serum MMP2 was highly expressed in patients with ARDS.?4?MiR-34a-5p maybe targeted regulation of MMP2 involved in the development of ARDS.?5?APACHE II score and miR-34a-5p elevation are independent risk factors for poor prognosis.