| Objective:Neoadjuvant chemotherapy(NAC)for breast cancer has been a standard treatment f or locally advanced breast cancer,and is also suitable for the vast majority of early patient s who requiring postoperative adjuvant chemotherapy.Patients who received PCR(comp lete pathological remission)after neoadjuvant chemotherapy had the better prognosis and disease-free survival.USP39 belongs to ubiquitous specified proteases(USPs)and is mai nly involved in mRNA cleavage and has the function of maintaining the integrity of the s pindle cleavage checkpoint,ensuring successful cell division.Previous studies have confi rmed that USP39 has a role in promoting breast cancer cell proliferation.However,it is ra rely studied,whether the expression USP39 can assess the effectiveness of Neoadjuvant c hemotherapy.In this study,an immunohistochemical study of preoperative puncture speci mens in breast cancer patients undergoing NAC was performed to analyze the relationshi p between the efficacy of NAC and USP39 protein expression,and to investigate whether the USP39 expression can predict the efficacy of NAC for breast cancer.Method:98 cases of breast cancer,who received neoadjuvant chemotherapy received in the br east disease health center of the Affiliated Hospital of Qingdao University from January 2016 to December 2017.Estimated in accordance with the chemotherapy effects on histolo gical system Miller And Payne,68 cases without neoadjuvant chemotherapy pathological complete remission(No-pCR)and 20 cases with neoadjuvant chemotherapy pathological complete remission(pCR).Visualization of the immunsystem,and attempt to discover th e USP39 protein expression in breast cancer patients before and after neoadjuvant chemot herapy for the treatment of chemotherapy.To analyze the difference of USP 39 protein ex pression in breast cancer patients with different chemotherapy efficacy and matched breas t cancer tissues before and after neo-adjuvant chemotherapy.to analyze the correlation be tween age,menstrual status,BMI,chemotherapy regimen and USP 39 protein expression,and further explore whether USP 39 protein expression can predict breast cancer neoadju vant chemotherapy pathological complete remission(pCR).Results:The age,menstrual status,BMI,chemotherapy regimen and other basic information of 98 patients with breast cancer were not significantly correlated with the expression of USP 39 protein in breast cancer tissue.At the same time,the clinic-pathological character istics of the subjects in the study area were analyzed.the results showed that USP 39 prot ein was highly expressed in the cancer tissues of PCR patients with neoadjuvant chemotherapy,but low expressed in no-PCR puncture specimens,and the difference was statistic ally significant.After neoadjuvant chemotherapy with USP 39 protein,the expression of USP 39 protein in breast cancer tissue decreased.There was no significant difference in t umor size,tumor tissue classification,molecular typing,ER?PR?her-2 expression betwe en the two groups and the expression of USP 39 protein(p > 0.05).The correlation betw een Ki67 expression and USP 39 expression was statistically significant(p < 0.001),and Ki67 expression was analyzed as a continuous variable rather than as a change in intensit y.the difference between Ki67 positive index expression and USP 39 expression in breast cancer tissues was statistically significant(p < 0.001).Conclusion:The expression of USP39 protein in breast cancer tissues decreased after neo-adjuva nt chemotherapy.The expression of USP39 was positively correlated with the expression of Ki67 in breast cancer tumor cell proliferation.Neoadjuvant chemotherapy for USP39 h igh expression breast cancer was more likely to achieve pCR.Therefore,the expression o f USP 39 protein can predict whether neoadjuvant chemotherapy of breast cancer can ach ieve pCR to some extent?... |
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