| Background Degenerative change of the intervertebral disc is a common clinical disease.It affects seriously the life quality of patients,and brings a heavy financial burden to them.Its complex pathogenesis is not entirely clear by now.It is of great significance to further explore the influencing factors related to its occurrence and development.It has been found that transforming growth factor-?1(TGF-?1)plays an important role in tissue and organ fibrosis and extracellular matrix synthesis of intervertebral disc,and wnt/beta-catenin signaling pathway is also associated with intervertebral disc degeneration.No comprehensive study of the two index in degenerative tissue of human lumbar intervertebral disc has been reported.This study investigated the relationship between?-catenin and TGF-?1 and human lumbar disc degeneration.Objective The main purpose of this paper is to study the expression of?-catenin and TGF-?1 in the degenerative lumbar intervertebral disc and the intervertebral disc of the control group,and to explore the relationship between the expression of?-catenin,TGF-?1 and degenerative intervertebral disc,and to provide a new theoretical basis for the occurrence,treatment and prevention of lumbar disc degeneration.Methods The expression of?-catenin and TGF-?1 was detected in 60 cases degenerative lumbar disc tissues and 10 cases of them in control group by immunohistochemical PV-9000 method.The difference of their expression between the two groups,the relationship to clinical parameters,Pfirrmann classification and the correlation between them were investigated.All data were processed by SPSS 22.0 statistical software.P<0.05 showed significant difference.Results Both?-catenin and TGF-?1 in degenerated lumbar disc tissue were localized in cytoplasm.The positive rate of?-catenin and TGF-?1 in degenerative lumbar disc tissue was significantly higher than that in the control group(X~2=14.378,16.727,P<0.01),and the positive intensity was significantly different(Z=-3.346,-3.863,P<0.01).The positive intensity of?-catenin and TGF-?1 was all related to the age of the patients(Z=-2.360,P<0.05;Z=-2.804,P<0.01),the disease duration(Z=-3.204,-3.159,P<0.01),and the Pfirrmann degree of degeneration of lumbar intervertebral discs(Z=-3.888,-4.478,P<0.001);but were not related to whether the patients had spinal stenosis(Z=-0.526,-1.719,P>0.05).The positive intensity of TGF-?1 was not related to the gender of the patients(Z=-0.838,P>0.05),while it of?-catenin was related to the gender of the patients(Z=-2.068,P<0.05).There was a significant positive correlation between?-catenin and TGF-?1 expression in degenerative lumbar disc tissues(r=2.965,P<0.01).Conclusion 1 The positive expression rate and intensity of?-catenin and TGF-?1 in degenerative lumbar intervertebral disc tissue were significantly higher than those in the control group,suggesting that the overexpression of?-catenin and TGF-?1 may be related to the degeneration of lumbar intervertebral disc.2 The positive intensity of?-catenin and TGF-?1 in Pfirrmann grade?-?degenerative lumbar disc tissues was higher than that in grade?,it also indicates that the overexpression of?-catenin and TGF-?1 may be related to the degree of degeneration of lumbar intervertebral discs.The expressive level of?-catenin and TGF-?1 may be correlated to the age and disease course of patients.3 The expression of?-catenin and TGF-?1 showed a significant positive correlation in the degenerated lumbar disc tissues,it implies that the overexpression of?-catenin and TGF-?1 may have a synergistic effect in the occurrence and development of lumbar disc degeneration.Combined detection of?-catenin and TGF-?1 may have important guiding significance in the occurrence,treatment and prevention of intervertebral disc degeneration. |
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