Transcriptomic Analysis Of Ovarian Abnormal Development In Mice Exposed To Bisphenol A At Adolescence

Bisphenol A ?BPA? has an estrogen effect that can disrupt endocrine.Many animal models and human epidemiology have linked BPA to the process of reproductive and post-embryonic development.However,little is known about the research that BPA affects adolescent mouse ovaries based on the transcriptome level.Therefore,we attempted to study ovarian development and transcriptomic characteristics of adolescent ICR female mice by oral BPA.Sixty female ICR mice of 4 w female ICR mice were randomly assigned to two groups:HBPA group and EC group,each group are thirty,respectively.The HBPA group was orally administered by gavage with BPA ?200 ng/kg/day? for 4 weeks.In the EC group,the same amount of corn oil was applied.Then,the estrus mouse serum were collected for BPA and 17?-estradiol ?E₂? hormone assay to explore whether BPA exposure affects estrogen secretion or not,while estrus ovarian were collected to histological observation and mRNA and protein expression levels of related genes.Finally,RNA-seq of ovary tissues was also performed and then analysis differential genes of GO function and KEGG pathway by using bioinformatic analysis.Results:1.No significant differences in the body weight were observed between the two groups during the experimental period,but ovarian matrix and interstitial gland appear hyperplasia,the uterus was hyperemia,the number of follicles decreased,the number of follicles increased,the corpus lute became bigger and the number of oocyte increased significantly by observing histology.2.BPA and E₂ level of serum samples in BPA group were higher than control group,and both immunohistochemistry and Western blotting showed an increase at the protein expression of ER?,ER?is the opposite.3.RNA-seq and functional annotation of mouse ovaries were performed to study the gene expression and function by biological methods and the results showed that the sequencing quality was pretty.Further,the results of GO term enrichment showed that DE genes are significantly enriched in MAPK cascade,cell differentiation,heart development and phosphate-containing compound metabolic process,the KEGG metabolic pathway is significantly enriched in the endocrine system category.Conclusion:Our results indicate that exposure to BPA in adolescent mice can cause damage to ovarian tissue structure,affect hormone secretion and gene expression levels,and disrupt the mouse endocrine system to induce premature or rapid ovarian development and can lead to mouse ovarian tissue.Changes in transcriptomics,including changes in gene function,provide the basis for a more in-depth study of the mechanisms by which BPA affects ovarian development.