| Objective: In order to investigate,the expression of costimulatory molecules B7H3 and B7H4 in T lymphoblastic lymphoma(T-LBL/ALL),and explore the relationship between their expression and clinicopathological features and prognosis.Methods: 1.Collected 100 cases of T-LBL/ALL archived wax blocks with complete clinical pathological data and follow-up records from Department of Pathology,Shanxi Cancer Hospital from 2001 to 2017.30 cases of lymph node reactive hyperplasia(LH)archived paraffin specimens were selected.2.Paraffin tissue samples from the experimental group and the control group were prepared into tissue pellets.Immunohistochemistry(En Vison method)was used to detect B7H3,B7H4,CD4 and CD8 in 100 T-LBL/ALL and 30 LH paraffin specimens.The protein expression of the indicators.3.Real-time RT-PCR was used to detect the expression of B7H3 mRNA and B7H4 mRNA in 50 T-LBL/ALL paraffin specimens and 30 LH paraffin specimens.4.SPSS 22.0 statistical analysis software was used to analyze the relationship between B7H3,B7H4 protein and mRNA expression and clinical case characteristics and prognosis of patients with T lymphoblastic lymphomaResults: 1.Immunohistochemistry results: B7H3 protein was mainly expressed in tumor cell membrane,macrophage membrane and some tumor-associated vascular epithelium in 100 cases of T-LBL/ALL paraffin,the positive rate was 23%(23/100)In 30 cases of LH paraffin tissue,it was mainly expressed in the germinal center of lymphoid follicles,and T cells were not expressed;B7H4 protein was mainly expressed in tumor cells in 100 cases of TLBL/ALL paraffin,and the positive rate was 54%(54/ 100),part of T cells can be expressed in 30 cases of LH paraffin,the positive rate is 26%.2.Real-time PCR results: The relative expression of B7H3 mRNA in 50 T-LBL/ALL paraffin tissues was higher than that in LH group(7.455vs3.675).However,the expression levels of B7H4 mRNA in the T-LBL/ALL group and the LH group were extremely low.3.Relationship between B7H3,B7H4 and T-LBL/ALL clinicopathological features: B7H3 protein expression in T-LBL/ALL paraffin tissue and whether there is B symptom,whether there is correlation in primary lymph nodes(P<0.05);B7H4 protein There was a correlation between the expression and whether there was a mediastinal widening and whether there was a bone marrow recidivism(P<0.05).4.The relationship between the expression and clinicopathological features of B7H3 and B7H4 and the prognosis of patients with T-LBL/ALL: The one-year survival rate of TLBL/ALL in B7H3 protein high expression group was significantly higher than that in low expression group,the difference was statistically significant(P =0.0081),suggesting that B7H3 protein is highly expressed,T-LBL/ALL patients have a better prognosis;B7H3 mRNA high expression group T-LBL/ALL one year survival rate is significantly lower than low expression group,the difference is statistically significant(P=0.002)High expression of B7H3 mRNA was suggested,and the prognosis of patients with T-LBL/ALL was poor.The annual survival rate of T-LBL/ALL in B7H4 protein high expression group was significantly lower than that in low expression group(P=0.0285),suggesting B7H4 protein.High expression,T-LBL/ALL patients with poor prognosis;B7H3,B7H4 protein coexpression analysis,found that B7H3 low expression and B7H4 high expression T-LBL/ALL one year survival rate is the lowest,the prognosis is the worst(P=0.011).IPI scores were associated with progression-free survival(P=0.0430).Multivariate Cox regression analysis: High expression of B7H3 mRNA was an independent risk factor for the prognosis of TLBL/ALL(β=1.304,HR=3.685,95% CI=1.563~8.691,P=0.003)Conclusion The expression of B7H3 and B7H4 is closely related to the stage of T-LBL/ALL tumor,mediastinal widening,hepatomegaly and prognosis.These suggest that B7H3 and B7H4 expression play an important role in the development of T-LBL/ALL.The expression of B7H3 and B7H4 proteins could not be completely reflected by mRNA levels,and it was speculated that B7H3 and B7H4 could affect the development of T-LBL/ALL by regulating other signaling pathways.We need to further study the mechanism of action of B7H3 and B7H4 in T-LBL/ALL,and provide new ideas for the treatment of T-LBL/ALL. |
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