A Study On The In Vitro Eukaryotic Expression Of Neuroglobin And Its Function In The Oxidative Defense During Glioma Progression

Neuroglobin,whose expression is induced by hypoxia and ischemia,is a nerve globin distributed mostly in central and peripheral nervous systems,functioning as a protector of neurons against oxidative stress.It has been reported that Neuroglobin involves in the development of cancers and neurodegenerative diseases,but the mechanisms underlie so far are not well defined.PPAR? is considered to be a key factor which suppresses cancers.Several clinical trials have exhibited PPAR? agonists interrupts the progression of malignant gliomas,contributes to the overoxidation-induced apoptosis in glioma cell lines.Interestingly,both Neuroglobin and PPAR? share a pattern of tumor grade related expression in gliomas,despite their trends are the exact opposite.In vitro experiments performed on U-87 MG cells to figure out the connection between Neuroglobin and PPAR?,as well as the mechanisms of Neuroglobin function shows that NGB is down-regulated by PPAR? agonists while a PPAR? antiagonist enhances NGB in glioma cells.Enhanced NGB protects glioma cells against oxidative stress by reducing ROS as it defends glioma cells from PPAR?-mediated oxidative damage.In addition,NGB is likely to contribute to the survival,multiplication and metastasis of glioma cells for promoting the phosphorylation of AKT.In all,all evidence display NGB plays a crucial role in glioma progression as an antioxidant and an transcriptional factor,demonstrating its potential of being a new molecular therapeutic targets in the treatment of glioma.