Effect Of Qingzao Jiufei Decoction On Metabolic Rate-limiting Enzymes And Metabolites Of Pentosephosphate Pathway And Krebs Cycle In Lung Cancer

Objective To discuss the effect of Qingzao Jiufei Decoction on Key rate-limiting enzymes and intermediate metabolites of pentosephosphate pathway,Krebs cycle in lung cancer,and then explore the influence and action mechanism of Qingzao Jiufei Decoction on lung cancer.Method 50 male C57BL6J mice were randomly divided into five group:model group,CTX group,and high dose group,medium dose group,low dose group of Qingzao Jiufei Decoction.Animal models were induced with the axillary injection of Lewis cells,the Qingzao Jiufei Decoction group started medication at molding two weeks before(11,5.5,2.75g·kg^-1·d^-1),CTX group(50mg·kg^-1,QOD)and model group?same volume of saline?started medication after molding.After 14d modeling,killed the mice and weigh tumor.We detected the enzymatic activity of Glucose-6-Phosphate Dehydrogenase?G6PD?,content of reactive oxygen species?ROS?,Cholesterol and Free Fatty Acids?FFA?by Elisa method.We detected the protein expression level of Aconitase 1?ACO-1?protein by Western-blot method.We detected the expressions of gp91phox and p22phox mRNA,which are subunit unit of NADPH oxidase and ATP citrate?pro-3S?-lyase?ACL?mRNA by Realtime-PCR method.Result1.Effect of the Qingzao Jiufei Decoction on tumor weight of Lewis-bearing miceCompared with the model group,the tumor weight of CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the tumor weight of high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the tumor weight of medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the tumor weight of low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.05?.The tumor inhibition rate of CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were94.7%?80.3%?57.2%?47.9%.The high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction have high inhibitory effect on tumor,in which the high,medium and low doses have a dose-effect relationship.2.Test results of related indexes by Elisa2.1 Effect of the Qingzao Jiufei Decoction on enzymatic activity of G6PDCompared with the model group,the enzymatic activity of G6PD in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01 or P<0.05?.Compared with the CTX group,the enzymatic activity of G6PD in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01 or P<0.05?.Compared with the high dose group of Qingzao Jiufei Decoction,the enzymatic activity of G6PD in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01 or P<0.05?.Compared with the medium dose group of Qingzao Jiufei Decoction,the enzymatic activity of G6PD in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.05?.2.2 Effect of the Qingzao Jiufei Decoction on content of ROSCompared with the model group,the content of ROS in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the content of ROS in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the content of ROS in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the content of ROS in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.2.3 Effect of the Qingzao Jiufei Decoction on content of CHOCompared with the model group,the content of CHO in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the content of CHO in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the content of CHO in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the content of CHO in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.2.4 Effect of the Qingzao Jiufei Decoction on content of FFACompared with the model group,the content of FFA in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the content of FFA in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the content of FFA in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the content of FFA in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.3.Test results of related indexes by Realtime-PCR.3.1 Effect of the Qingzao Jiufei Decoction on expression of gp91phox mRNACompared with the model group,the expression of gp91phox mRNA in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the expression of gp91phox mRNA in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the expression of gp91phox mRNA in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the expression of gp91phox mRNA in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.3.2 Effect of the Qingzao Jiufei Decoction on expression of p22phox mRNACompared with the model group,the expression of p22phox mRNA in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the expression of p22phox mRNA in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the expression of p22phox mRNA in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the expression of p22phox mRNA in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.3.3 Effect of the Qingzao Jiufei Decoction on expression of ACL mRNACompared with the model group,the expression of ACL mRNA in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01?.Compared with the CTX group,the expression of ACL mRNA in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the expression of ACL mRNA in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the medium dose group of Qingzao Jiufei Decoction,the expression of ACL mRNA in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.4.Test results of related indexes by Western-blot:Effect of the Qingzao Jiufei Decoction on the protein expression level of ACO-1 proteinCompared with the model group,the protein expression level of ACO-1 protein in CTX group,high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were decreased obviously,the difference was significant?P<0.01 or P<0.05?.Compared with the CTX group,the protein expression level of ACO-1 protein in high dose group,medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01?.Compared with the high dose group of Qingzao Jiufei Decoction,the protein expression level of ACO-1 protein in medium dose group and low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.01 or P<0.05?.Compared with the medium dose group of Qingzao Jiufei Decoction,the protein expression level of ACO-1 protein in low dose group of Qingzao Jiufei Decoction were increased obviously,the difference was significant?P<0.05?.Conclusion1.Qingzao Jiufei Decoction can decrease the tumor weight of Lewis-bearing mice.2.Qingzao Jiufei Decoction maybe can inhibit the expression of NADPH Oxidase,reduce the content of ROS,and then it can inhibit the expression of G6PD,which can reduce the metabolism of pentose phosphate as a raw material for DNA Synthesis in lung cancer cells.and inhibit the energy metabolism.3.Qingzao Jiufei Decoction maybe can inhibit the expression of ACO-1 and ACL,reduce the supply of tricarboxylic acid cycles to cellular synthetic materials FFA and CHO,and inhibit the energy metabolism.