The Establishment Of The In-vitro Model Of Tubal Pregnancy Implantation

Objective:We establish the in-vitro model of tubal pregnancy implantation throuth the co-culture of embryo villus cells(JAR)and oviductal epithelial cells(OE-E6/E7).Methods:We divided the experiment into 3 groups: embryo villus cells(JAR)group,oviductal epithelial cells(OE-E6/E7)group and the co-culture of embryo villus cells(JAR)and oviductal epithelial cells(OE-E6/E7)group.Each group had 6 parallel experiments.We made 2 scales between JAR and OE-E6/E7: 1:6 and 1:12.Each scale had 6 co-culture groups.At the same time,we set JAR groups into 1:6 and 1:12(compare with OE-E6/E7 cells).And also,Each scale had 6 parallel groups? The method of cell counting was cell-count boards.We digested and blew JAR and OE-E6/E7 group into sigle cells by pancreatic enzymes.And then we used the cell-count boards.The dead cells were collected by centrifuging the supernate.We left 100-200 ul liquid and counted by cell-count boards.The co-culture groups were counted under microscope.At the same time,We checked ICAM1 by Flow Cytometry,and analyzed the changes of different proteins by protein-chip technique.Results:The adhesion rates of the co-culture groups which cultured 72 hours were higher than the 48 hours cultured groups.While,there were no statistical differences between the 1:6groups and the 1:12 groups.Conclusions:We successfully establish the in-vitro model of tubal pregnancy implantation throuth the co-culture of embryo villus cells(JAR)and oviductal epithelial cells(OE-E6/E7)under the scales 1:6 and 1:12.