| Objective:To explore the molecular biologic mechanism of posttraumatic knee extrinsic contractures of rabbit.Methods:A model of posttraumatic knee extrinsic contracture of rabbit was created to be studied the differentially expressed genes.The left knees of three mature New Zealand rabbits?12-18 months old?that were operated to result in a stable intra-articular fracture and immobilized,served as trauma group,and the right knees without any operations served as control group.The gene expression profiles of joint capsules were analyzed with Agilent rabbit whole genome microarray.Differentially expressed genes were screened by comparing gene expression profiles between trauma and control group.Among those differentially expressed genes,Matrix metalloproteinase 2?MMP2?,Actin alpha 2?Acta2?,and Keratinocyte growth factor?KGF?gene,which related to fibrosis,were selected for verification by real-time reverse transcription polymerase chain reaction?Real-time RT-PCR?analysis.Results:Comparing to normal capsules,the contracted capsules had ninety differentially expressed genes,of which,twenty-one were up-regulated and sixty-nine were down-regulated.These genes were associated with regulation of response to external stimulus,regulation of lipopolysaccharide-mediated signaling pathway,regulation of response to biotic stimulus etc.The results of Real-time RT-PCR were consistent with the results of the microarray that MMP2,Acta2,and KGF was up-regulated.Conclusions:There are differentially expressed genes in the joint capsules of rabbit knee of posttraumatic joint extrinsic contractures compared with the normal knees.The results of increased expression of MMP2 and Acta2 genes were consistent with the reports about human beings[1,2],while the increased expression of KGF gene has not been mentioned by the authors.Post-traumatic knee joint extrinsic contractures may be associated with increase expression of MMP2 and Acta2 gene,and the KGF gene need for further research in Human joint contractures. |
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