The Role Of T Lymphocyte In The Formation Of Keloid Inflammation And Its Mechanism Of Regulation:A Preliminary Study

Objectives To investigate the characteristics of T lymphocytes among keloid patients in comparation with general population,and explore the function and regulation mechanism of T lymphocytes in the inflammation of keloid scar.Methods Medical history and samples,including venous blood and tissue samples,were collected from health subjects and keloid patients.By using density gradient separation method,peripheral blood mononuclear cells were isolated from whole blood,and by collagenase digestion method,the primary uncultured cells were isolated from tissue.Through the BD Bioscience spectrum platform,designed flow cytometry color scheme was used to compare the phenotype and function of memory T cells and regulatory T cells in peripheral blood mononuclear cells and tissue cells between keloid patients and health subjects,and its correlation with clinical symptoms.Results This study has found,in the first time,that most of T lymphocytes in keloid scars had the memory phenotype which showed distinct characteristics with these in normal skin,including increased IFN-γand reduced TNF-αproduction in CD4⁺T cells along with increased number and CD103 expression in CD8⁺T cells.Furthermore,study also identified decreased CD25 and CTLA-4 expression and IL-10 production in regulatory T cells from keloid scar.Finally,this study has found a decrease in the number of CD4⁺CD25^highFOXP3⁺regulatory T cells in peripheral blood of patients with multiple keloid scar.Conclusions This study confirmed that there were abnormalities in the memory T lymphocytes in the tissue of keloid patients compared with normal skin,and this abnormality may due to the dysregulation of regulatory T cells in tissue or peripheral blood.