The Study Of CD28 Costimulatory Superfamily And CD4～+ Regulatory T Cells In Immune Pathogenesis Of Acquired Aplastic Anemia

Objective:To explore the possible immune pathogenesis of CD28 costimulatory superfamily and CD4+ regulatory T cells in acquired aplastic anemia.Methods:1. By flow cytometry, the percentages of CD28, ICOS, CTLA-4 and PD-1 expressing in the membranes of CD4+T cells, the levels of IFN-γ, IL-4, TGF-β1 and IL-10 in the cytoplasm of CD4+T cells and the counts of CD4+ CD25+regulatory T cells in bone marrow were detected in 23 patients with AA at active phase, 10 patients with AA at recovery phase, 15 patients with IDA respectively.2. The relationships between the costimulators in the membranes of CD4+T cells, CD4+IFN-γ+, CD4+IL-4+, CD4+TGF-β1+, CD4+IL-10+, CD4+CD25+ regulatory T cells and ANC were evaluated.Results:1. The percentage of CD28 expressing by CD4+T cells in bone marrow with AA at active phase was significantly higher than that with IDA controls(p=0.028<0.05), but the percentage of CD28 was lower significantly with AA at recovery phase than at active...