Studies On The Chemical Constituents Of Clausena Dunniana Var.robusta And Xylopia Vielana Pierre

As a natural drug researcher,one of our main tasks is to extract and separate potential active compounds from natural products as pro-drugs for further research.Nowadays,there are two main methods for screening target plants.One way people select is further studying the bioactive plants through active screening.The other one is studying the possible novel structure of the plants from same Genus after some literature investigations.The first target plant,Clausena dunniana var.robusta,was selected by screening the anti-inflammatory activity screening,which showed high activity against the NF-?B.Moreover,through literature research,we found that the Xylopia vielana Pierre,which is one species from the Xylopia,are full of sesquiterpene dimers,that are relatively rare in nature products.Therefore,this plant is selected as the second target plant.It is a kind of macrophanerophytes with branches dark brown and densely rounded lenticels,which can grow up to 20 meters' tall.ItsBranchlets are coverd by velutinous when it is young and disappear with growing up.It is grown in Hunan,south of Guangxi and other places in our country.During the process of systematical extraction,isolation and identification of these two plants,we applied a variety of chromatographic separation technologies,including silica gel column chromatography,Sephadex LH-20 gel column chromatography,medium pressure preparation,preparative HPLC and preparative thin layer chromatography.After obtaining different single compounds,we also used a variety of spectral identification techniques,such as 1H-NMR,13C-NMR,2D-NMR,IR,CD,EI-MS,HRESI-MS and XRD to identify the absolute structures of these compounds.Finally,we got 24 compounds from the Clausena dunniana var.robusta.Four of these compounds were new compounds.All these compounds were phenylpropanoid glycosides(HP-1,HP-2,HP-3,HP-4).We also got 14 compounds from the X.vielana Pierre.Eight of them were new compounds,including 5 new sesquiterpene dimers(M-1-M-5)and 3 new alkaloids(M-7-M-9)In our research,for the compounds from the C.dunniana va.robusta,we tested the inhibitory effect on TNF-a induced NF-?B activity of the isolated compounds in HeLa cells.As a result,HP-17 exhibited inhibition of NF-?B activation.In addition,HP-1-HP-3,HP-6-HP-14 and HP-17 showed inhibition of Klebsiella pneumoniae and HP-5,HP-16 exhibited inhibition of Enterococcus faecalis.What's more,for the compounds from the.vielana Pierre,M-4 and M-10 showed activity of inhibiting the NO release.The IC50 of these two compounds are 4.59±1.03 ?M and 1.39±1.49 ?M respectively.