| Objective The treatment that performed with Mongolian medicine warm acupuncture at the "hip point" of the chronic sciatic nerve constrictive injury model(CCI)rats,observe the influence of Mongolian medicine warm acupuncture on behavior and inflammatory cytokines of rats with chronic sciatic nerve constrictive injury(CCI).In order to explore the mechanism of Mongolian medicine warm acupuncture in the treatment of sciatic neuralgia.Method 90 male SD rats with body weight(200±40)g were selected and randomly divided into blank group,sham-operated group,model group,Mongolian medicine warm acupuncture group,Mongolian medicine acupuncture group and Western medicine group,with 15 rats in each group.CCI animal models were made by Bennettxie method.Among these groups,in the blank group,no model was established;In the sham-operated group,only the sciatic nerve was exposed without ligation;in the other groups,the right sciatic nerve was ligated.The treatment began 15 days after the success of the model.The blank group,model group and sham-operated group were not treated,while the Mongolian medicine warm acupuncture group was treated by acupuncture "hip point".The rats were treated with warm acupuncture once a day for 15 minutes for 15 days.Mongolian Medicine Acupuncture Group: Acupuncture at "hip point".Then the rats fixed with self-made fixator and treated with "hip point" acupuncture once a day for 15 minutes for 15 days.The western medicine group was given ibuprofen(0.1g/Kg)for 15 days.During the treatment,Body weight and general behavior of rats were observed 4d,12 d,20d and 28 d after operation,and the behavioral evaluation was carried out by measuring the threshold of mechanical stimulation(MWT)and the latent period of heat-shrink-leg reflex(TWL),open field experiment and elevated cross maze.Within 6h after the last treatment,the rats were anesthetized by intraperitoneal injection of 10% chloral hydrate solution(0.35/100 g for each rat),and the abdominal aorta was prepared for blood collection after anesthesia.Serum levels of interleukin-6(IL-6),interleukin-10(IL-10),interleukin-1beta(IL-1beta),interleukin-2(IL-2)and other cytokines were measured by enzyme-linked immunosorbent assay(ELISA).Result 1.General Behavior: At the initial stage after successful modeling,the rats showed mild spontaneous foot lift or repeated licking and biting of the affected side(at the modeling site),or foot swing,or interstitial claudication of the right limb.With the extension of modeling time(the peak of pain),the rats showed changes in autophagy,right toe or surgical site,self-defense,irritability,aggression and other neuropathic behaviors,as well as local skin and muscle tension.Compared with the model group,the rats in the Mongolian medicine warm acupuncture group,the Mongolian medicine acupuncture group and the western medicine group showed significantly less autophagy,right toe or surgical site,self-defense,irritability,aggression and other neuropathic behaviors,as well as changes in local skin and muscle tone.2.The mechanical stimulation threshold(MWT),thermal leg reflex latency(TWL),open field experiment,elevated cross maze and other behavioral tests showed that there were significant differences between the model group and the normal group(P<0.05);Compared with the model group,there were significant differences in the Mongolian medicine warm acupuncture group,the acupuncture group and the western medicine group(P < 0.05).3.The levels of interleukin-6(IL-6),interleukin-1beta(IL-1beta),interleukin-2(IL-2)and other cytokines in rat serum were significantly different from those in the normal group(P<0.01).Compared with the model group,there were significant differences in Mongolian medicine warm acupuncture group,Mongolian medicine acupuncture group and western medicine group(P<0.01).The levels of interleukin-10(IL-10),were significantly different from those in the normal group(P<0.01).Compared with the model group,there were significant differences in Mongolian medicine warm acupuncture group,Mongolian medicine acupuncture group and western medicine group(P<0.01).Conclusion 1.This study confirmed that the Mongolian medicine warm acupuncture at the "hip point" of the rats with chronic sciatic nerve constrictive injury(CCI)improved the behavior of the rats with chronic sciatic nerve constrictive injury(CCI)by improving the pain threshold,blood circulation and relieving spasm.2.This study confirmed the anti-inflammatory and analgesic effects achieved by inhibiting the contents of inflammatory cytokines interleukin-6(IL-6),interleukin-10(IL-10),interleukin-1beta(IL-1beta),interleukin-2(IL-2)and tumor necrosis factor(TNFa)in the serum of the "hip point" of the chronic sciatic nerve constrictive injury model(CCI)rats that treated by Mongolian medicine warm acupuncture.3.The Mongolian medicine warm acupuncture therapy through the acupuncture and the warm heat stimulation,to the nerve-endocrine-immune network regulation to play the role.4.In the model of chronic sciatic nerve constrictive injury model(CCI)rats,the "hip point" was treated by Mongolian medicine warm acupuncture,and the therapeutic purpose was achieved by regulating three roots and improving Qi and blood circulation,toning the white veins,and improving the functions of various organs and the body immunity.Mongolian medicine warm acupuncture therapy is simple,safe,with few side effects,multiple channels and comprehensive treatment,which can be further promoted in clinical practice. |
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