Effect Of MiR-194-5p On Proliferation,Migration And Activation Of LX-2 Cells

Objectives To investigate the effect of miR-194-5p on the proliferation,migration and activation of LX-2 cells and the possible molecular mechanisms.Methods 1 Quantitative real-time PCR was used to detect the expression of miR-29a-3p,miR-194-5p,miR-22-3p in LX-2 cells with the treatment of TGF-?₁.2 The experiment was divided into mimics NC group,miR-194-5p mimics group,inhibitor NC group,miR-194-5p inhibitor group,transfection of LX-2 cells by liposome,qRT-PCR detection of miR-194-5p Relative expression level.3 The effection of miR-194-5p on the proliferation of LX-2 cells was detected by CCK-8 assay and colony formation assay.4 To assess the function of miR-194p on the migration of LX-2 cells by healing test.5 Quantitative real-time PCR was used to detect the effect of miR-194-5p on the relative expression of?-SMA?Collagen I mRNA in LX-2 cells.6 The candidate target gene of miR-194-5p was predicted with bioinformation softwares.7 Quantitative real-time PCR was used to detect the effect of miR-194-5p on the relative expression of wnt5a mRNA in LX-2 cells.Results 1 qRT-PCR results showed that,compared with the Ctrl group,the relative expression levels of miR-29a-3p,miR-194-5p and miR-22-3p in the TGF-?₁ stimulation group were decreased,and the differences were statistically significant?P<0.01?.2 qRT-PCR detected the changes in the relative expression level of miR-194-5p after transfection,and the results showed that,compared with the mimics NC group,the relative expression level of miR-194-5p in the mimics group was increased,and the difference was statistically significant?P<0.01?.Compared with the inhibitor NC group,the relative expression level of miR-194-5p in the miR-194-5p inhibitor group was decreased,and the difference was statistically significant?P<0.01?.3 The results of CCK-8 experiment showed that,compared with the mimics NC group,the proliferation ability of miR-194-5p mimics group was decreased,and the difference was statistically significant?P<0.01?.Compared with the inhibitor NC group,the proliferation ability of miR-194-5p inhibitor group was increased,and the difference was statistically significant?P<0.01?.The colony formation experiment results showed that,compared with the mimics NC group,the colony formation ability of miR-194-5p mimics group was weakened,and the difference was statistically significant?P<0.05?.Compared with the inhibitor NC group,the colony formation ability of miR-194-5p inhibitor group was enhanced,and the difference was statistically significant?P<0.05?.4 Scratch test results showed that,compared with the mimics NC group,the migration ability of miR-194-5p mimics group was weakened,with statistically significant difference?P<0.05?.Compared with the inhibitor NC group,the migration ability of miR-194-5p inhibitor group was enhanced,and the difference was statistically significant?P<0.05?.5 the effect of miR-194-5p on the relative expression levels of?-SMA and Collagen I mRNA in the mimics NC group was detected by qRT-PCR test,and the results showed that the relative expression levels of?-SMA and Collagen I mRNA in the miR-194-5p mimics group were decreased,showing a statistically significant difference?P<0.01?.Relative expression levels of?-SMA and Collagen I mRNA in miR-194-5p inhibitor group showed increased when compared with inhibitor NC group?P<0.01?.6 Combined with bioinformatics database and gene chip,it was predicted that wnt5a was a candidate target gene of miR-194-5p.7 The effect of miR-194-5p on the relative expression level of wnt5a mRNA was detected by qRT-PCR.The experimental results showed that compared with the mimics NC group,the relative expression level of wnt5a mRNA in the mimics group was decreased,and the difference was statistically significant?P<0.01?.Compared with the inhibitor NC group,the relative expression level of wnt5a mRNA of miR-194-5p inhibitor group was increased,and the difference was statistically significant?P<0.01?.Conclusions miR-194-5p may attenuate the proliferation,migration and activation of LX-2 cells by the candidate target gene wnt5a.Figure 16;Table 1;Reference 116...