| Background: Acute pancreatitis is a kind of fierceness of common diseases,liver and gallbladder surgery in the heavier conditions when still can cause systemic inflammatory response syndrome,due to the liver and pancreas of special structure,the course of acute pancreatitis can appear early liver damage,and damage to the liver and can cause and aggravate the other viscera damage apart.In recent years,due to the advantages of multidirectional differentiation and low immunogenicity of adipose mesenchymal stem cells,as well as the advantages of easy collection,strong plasticity and strong proliferation ability,many domestic and foreign scholars have conducted a lot of studies on them.At present,the treatment of acute pancreatitis related organ injury by in vivo transplantation of ADSCs has become one of the research hotspots.Objective: To investigate the mechanism of adipose mesenchymal stem cells(ADSCs)in the treatment of liver injury induced by acute pancreatitis.Methods: 1.30 healthy male SD rats were randomly divided into normal control group(sham operation group),model group(acute pancreatitis liver injury group)and experimental group(fat mesenchymal stem cell transplantation group),10 rats in each group.The liver injury model group of acute pancreatitis and the fat mesenchymal stem cell transplantation group were used to establish the acute pancreatitis model of rats by retrograde injection of 3% sodium sulfonycholic acid through the bile duct,and the serum ALT and AST indexes were detected 6 hours after the injection to demonstrate the liver injury.In the control group,only the duodenum and pancreas were turned open.Allogeneic rat adipose mesenchymal stem cells were obtained 1 week before the experiment.After successful modeling,ADSCs transplantation was performed on rats in the experimental group,and the number of adipose mesenchymal stem cells in logarithmic growth period was about 5 10 and 6 by tail vein transplantation.The sham group and the pancreatitis model group received the same dose of saline injection.2.After successful modeling,tail venous blood of the three groups of rats at 24 h,48h and72 h after modeling was taken,and the ALT and AST contents in serum of the rats were detected.Histopathological changes of pancreas and liver were observed by HE staining.The levels of TNFand il-6 in mesenchymal stem cells were determined by ELISA at 24 h,48h,and 72 h after transplantation.Results: All rats in the control group survived 72 h after transplantation of adipose mesenchymal stem cells into the caudal vein.Two and three rats died in SAP group at 24 h and 48 h.Two rats in the ADSCs group died after 48 h,and the difference between the two groups was not statistically significant(P>0.05).The levels of ALT,AST,TNF-and il-6 in serum of the model group and the experimental group were significantly higher than those of the control group(P<0.05).Compared with the model group,the serum ALT,AST,TNF-and il-6 expression levels of the experimental group were significantly decreased(P<0.05).By high power optical microscope to observe the liver pathological HE staining smear results show that: compared with control group,pancreatitis between liver injury model group and group mesenchymal stem cell transplantation in the rat liver tissue injury situation is more serious,but between mesenchymal stem cell transplantation group of rat liver tissue degeneration,necrosis and inflammatory cells infiltration condition compared with model group significantly reduce.Conclusion: Transplantation of adipose-derived mesenchymal stem cells via caudal vein can alleviate liver injury induced by acute pancreatitis by inhibiting the expression of TNF-,il-6 and alleviating the inflammatory response in liver tissue. |
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