A Preliminary Study Of The Correlation Between MiR-26a-1,MiR-146a And MiR-199a-1 And Acute Myocardial Infarction

Background and aim: Acute myocardial infarction(AMI)was considered to be one of the major causes of morbidity and mortality worldwide.A great number of patients with evolving myocardial infarction die before they reach hospital to receive medical treatment.In order to reduce mortality and improve prognosis of AMI,early and accurate diagnoses is needed for effective treatment.The currently preferred diagnostic biomarkers for AMI are cardiac troponin I and T(c Tn I and c Tn T).However,false positive results with elevated c Tn could associate with disease such as heart failure,chronic kidney diseases and sepsis,especially in elderly patients.In addition,as a marker of cell injury,cardiac troponin T(c Tn T)does not peak until symptom onset.Therefore,additional biomarkers for AMI has been sought in order to improve diagnosis accuracy and also to help risk stratification.In order to manage the acute myocardial infarction outbreaks,novel accurate biomarkers for risk prediction are needed.Circulating micro RNAs(mi RNAs)may act as diagnostic and prognostic biomarkers for cardiovascular events.Former studies have shown that mi R-26 a participates in cardiovascular disease by controlling endothelial cell growth,angiogenesis,and LV function post-MI;Mi R-27 a participates in the regulation of cardiomyocyte apoptosis by targeting interleukin-10 pathway;Mi R-30 d is expressed in cardiomyocytes and heart tissues and regulates cardiomyocyte pyroptosis by directly targeting foxo3a;Mi R-146 a,which is located at the human chromosome 5q33,is also reported to be related to coronary artery disease(CAD);Mi R-199 a which is principally expressed in cardiomyocytes damages autophagy and induces cardiac hypertrophy by activating m TOR,and the expression of mi R-199 a is previously reported to be up-regulated by10-fold in hypertrophy hearts;Mi R-423-5p could be released by thrombin-stimulated platelets during myocardial infarction and be taken up by endothelial cells.However,the diagnostic significance of these mi RNAs in AMI needs further investigation.In this study,we aim to evaluate the plasma expression levels of mi R-26a-1,mi R-27 a,mi R-30 d,mi R-146 a,mi R-199a-1 and mi R-423 in AMI patients before and after percutaneous coronary intervention(PCI)by comparing with health controls.The purpose of this study was to investigate the relationship between mi R-26a-1,mi R-27 a,mi R-30 d,mi R-146 a,mi R-199a-1,mi R-423 and acute myocardial infarction,and to further study whether micro RNA-26a-1 can become a new biomarker of acute myocardial infarction.Methods: This study aimed to determine the possibility of circulating mi RNAs used as biomarkers for AMI and their dynamic expression levels before and after percutaneous coronary intervention(PCI)in patients.Circulating mi R-26a-1,mi R-27 a,mi R-30 d,mi R-146 a,mi R-199a-1 and mi R-423 were selected and validated in 31 AMI patients and27 matched controls by quantitative real-time PCR.All statistical tests were double-tailed,and P < 0.05 was considered to have statistical significance.Results: The expression levels of plasma mi R-26a-1,mi R-146 a and mi R-199a-1 were significantly increased in AMI patients.Receiver Operating Characteristic(ROC)analysis indicated that mi R-26a-1,mi R-146 a and mi R-199a-1 showed considerable diagnostic efficiency for predicting AMI.Moreover,we demonstrated that the combination of mi R-26a-1,mi R-146 a and mi R-199a-1 facilitated AMI diagnosis.Conclusions: Our findings suggest that circulating mi R-26a-1,mi R-146 a and mi R-199a-1 have the potential to be used as biomarkers for AMI diagnosis.