| PurposeNonalcoholic fatty liver disease(NAFLD)is the deposition of fat in liver cells,except for the deposition of fat in liver cells caused by excessive drinking and other factors that damage the liver.NAFLD disease spectrum includes nonalcoholic fatty liver(NAFL),nonalcoholic steatohepatitis(NASH),cirrhosis and hepatocellular carcinoma.With the change of living standard,working style and dietary structure,the incidence of NAFLD in China is increasing year by year.Patients with NAFLD usually have certain characteristics of metabolic syndrome(MetS),especially the risk factors for multiple coronary artery disease(CAD).A number of studies have confirmed that patients with NAFLD have a predisposition to CAD,and CAD is considered to be one of the leading causes of death in patients with NAFLD.In recent years,in-depth research on lipid metabolism has found that it plays an important role in the occurrence and development of NAFLD and CAD.The(35)-6 desaturase encoded by fatty acid desaturase 2(FADS2)is a key enzyme in the synthesis of polyunsaturated fatty acids(PUFAs).However,there is still a lack of research on FADS2 gene polymorphism in patients with NAFLD and CAD.Therefore,this study selected healthy people,NAFLD patients,and NAFLD patients with CAD in Chinese Han population as subjects to explore the correlation between FADS2 rs3834458 polymorphism and NAFLD with CAD.Method(1)This study included 149 patients in the control group,113 patients in the NAFLD group,and 75 patients in the NAFLD combined with CAD(NAFLD+CAD)group.All subjects were fasted for 12 hours,and 2 mL of venous blood was collected in the EDTA anticoagulation tube on the morning of the next day.After the plasma and blood cells are separated by centrifugation,they are stored separately in a refrigerator at-80 °C.(2)The level of FADS2 in serum of healthy control group,NAFLD group and NAFLD+CAD group were detected by enzyme-linked immunosorbent assay(ELISA).(3)Genotypic distribution of FADS2 rs3834458 in three groups of subjects was detected by polymerasechain reaction(PCR)and MALDI-TOF mass spectrometry.(4)The study collected general clinical characteristics such as age,gender,and body mass index(BMI)of all subjects and biochemical indicators such as blood glucose(GLU),total bilirubin(TBIL),and triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),alanine aminotransferase(alanine)Aminotransferase,ALT),aspartate aminotransferase(AST)alkaline phosphatase(ALP),Gama-glutamyl transferase(GGT).(5)Finally,all data were analyzed by SPSS 25.0 statistical software to comprehensively analyze the correlation between FADS2 rs3834458 gene polymorphism and NAFLD combined with CAD.P<0.05 was considered as statistically significant differenceResults(1)There was no significant difference in age,gender,TBIL and AST between the three groups(The P values were 0.927,0.442,0.242 and 0.141).The BMI,GLU,TC,LDL-C,ALT and GGT in the NAFLD group were significantly higher than those in the healthy control group(P<0.05);the BMI,GLU,TG,TC,LDL-C,ALT,GGT and ALP in the NAFLD+CAD group were significantly higher than those in the healthy control group(P<0.05);the BMI,TG,LDL-C,HDL-C,ALT and GGT in the NAFLD+CAD group were significantly lower than those in the NAFLD group(P<0.05);the HDL-C of NAFLD+CAD group was significantly lower than that of healthy control group and NAFLD group(P<0.05).(2)The serum FADS2 concentration in NAFLD+CAD group was significantly higher than that in NAFLD group(P<0.05)and healthy control group(P<0.05).There was no significant difference in serum FADS2 concentration between the control group and the NAFLD group(P=0.771).(3)Genotype distribution(?2=2.459,P=0.652)and allele frequency(?2=0.688,P=0.709)between the three groups were not statistically significant different.(4)Logistic regression analysis showed that BMI,GLU and TC(P<0.05)were associated with CAD combination with NAFLD.However,the T allele(P=0.483)was not associated with NAFLD combined with CAD(OR: 1.62,95% CI: 0.422-6.180).Conclusions(1)BMI,GLU,and TC are independent risk factors for NAFLD combined with CAD.(2)Serum FADS2 concentration was positively correlated with NAFLD CADsusceptibility,while FADS2 rs3834458 polymorphism was not significantly correlated with NAFLD combined with CAD susceptibility.SignificanceWe explored the correlation between serum concentration of FADS2 and its genetic polymorphisms and the susceptibility of NAFLD to CAD,which helps to elucidate the pathogenesis of NAFLD and CAD from the perspective of genetics.This is also of great significance for the identification of NAFLD associated CAD susceptibility genes.Moreover,it also helps to explore the treatment of gene therapy and make treatment more individualized. |
