| Objectives By detecting the expression levels of interleukin-1?,IL-1? and Tumour necrosis factor-?(TNF-?)in the serum of patients with IBS,to analyze the relationship between IL-1?,TNF-? and anxiety and depression,and to explore the possible role of IL-1? and TNF-? in the pathogenesis of IBS.Methods 30 cases of IBS patients admitted to hebei provincial people's hospital from May 2018 to January 2019 and meeting Rome IV diagnostic criteria were included,and 15 healthy subjects(group N)were included.Through SAS(self-rating anxiety scale)and SDS(self-rating depression scale),IBS patients were divided into the following two groups: IBS patients with anxiety and/or depression(group S,n=22),and patients with simple IBS(group E,n=8).Relevant clinical data were recorded and serum samples were collected.The expression levels of IL-1? and TNF-? in serum were detected by enzymelinked immunosorbent assay(ELISA);one-way analysis of variance was used to compare the differences of SAS score,SDS score and IL-1? level in N,E and S groups;nonparametric test was used to compare the difference of TNF-? levels in N,E and S groups;Pearson correlation analysis was used to test the correlation between IL-1? levels in S group and SAS and SDS scores,respectively;Spearman correlation analysis was used to test the correlation between TNF-? levels and SAS and SDS scores,respectively.Results 1 Comparison of SAS and SDS scores in N,E and S groups.1.1 The SAS and SDS scores of group E were(41.686±4.920)points and(44.817±3.718)points,respectively;the scores of SAS and SDS in group N were(42.980±11.339)points and(42.333±10.242)points,respectively;there were no significant differen ces in the scores of SA and SDS between group E and group N(P>0.05).1.2Th e SAS and SDS scores of the S group were(52.227±5.807)points and(54.417±4.243)points respectively;the SAS and SDS scores of group E were(41.686±4.920)points and(44.817±3.718)points,respectively;the scores of SAS and SDS in gr oup S were higher than those in group E,and the difference was statistically sign ificant(P<0.05).1.3 The SAS and SDS scores of the S group were(52.227±5.807)points and(54.417±4.243)points respectively;the scores of SAS and SDS in gr oup N were(42.980±11.339)points and(42.333±10.242)points,respectively;the sc ores of SAS and SDS in group S were higher than those in group N,and the dif ference was statistically significant(P<0.05).2 Serum levels of IL-1? and TNF-? in N,E and S groups were compared.2.1 Serum IL-1? and TNF-? levels in group E were(36.596±3.858)pg/ml and [7.215(6.726,8.666)]pg/ml,respectively;serum IL-1? and TNF-? levels in N group were(25.253±3.820)pg/ml and [5.959(4.784,6.916)]pg/ml,respectively;serum IL-1? and TNF-? levels in group E were higher tha n those in group N,and the difference was statistically significant(P < 0.05).2.2Serum IL-1? and TNF-? levels in group S were(43.991±7.421)pg/ml,[8.897(7.186,10.476)]pg/ml,respectively;serum IL-1? and TNF-? levels in group E were(36.596±3.858)pg/ml and [7.215(6.726,8.666)]pg/ml,respectively;serumIL-1? and TNF-? levels in group S were higher than those in group E;and the difference was statist ically significant(P < 0.05).2.3 Serum IL-1? and TNF-? levels in group S were(43.991±7.421)pg/ml,[8.897(7.186,10.476)]pg/ml,respectively;serum IL-1? and TNF-? levels in N group were(25.253 ±3.820)pg/ml and [5.959(4.784,6.916)]pg/ml,re spectively;serum IL-1? and TNF-? levels in group S were higher than those in group N;and the difference was statistically significant(P < 0.05).3 Correlation b etween serum IL-1?,TNF-? levels and SAS and SDS scores in group S.3.1 Seru m IL-1? level was positively correlated with SAS score(r = 0.781,P < 0.05).3.2 Serum IL-1? level was positively correlated with SDS score(r = 0.633,P < 0.05).3.3 Serum TNF-? level was positively correlated with SAS score(r= 0.730,P < 0.05).3.4 Serum TNF-? level was positively correlated with SDS score(r= 0.644,P < 0.05).Conclusions The expression levels of IL-1? and TNF-? in serum of IBS patients were significantly higher than those of healthy subjects,and the expression levels of IL-1? and TNF-? in serum of IBS patients with anxiety and/or depression were significantly higher than those of IBS patients alone.Serum IL-1?,TNF-? levels were positively correlated with SAS and SDS scores in patients with IBS with anxiety and/or depression,suggesting that IL-1? and TNF-? may be involved in the pathogenesis of IBS;anxiety and depression may aggravate the clinical symptoms of IBS by affecting the expression of serum IL-1? and TNF-?,triggering an inflammatory response.Figure 0;Table4;Reference 46... |
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