Correlation Analysis Of Serum ?-synuclein,? Tumor Necrosis Factor And Interleukin-6 In Patients With Parkinson's Disease

Objective:The abnormal aggregation of alpha synuclein(SNCA)and neuroinflammation play a dominant role in the pathogenesis of Parkinson's disease(PD).With the in-depth study,it is found that the cytotoxicity induced by the abnormal aggregation of SNCA may be mediated by neuroinflammation.Currently,most of the studies on them are carried out alone or limited to basic laboratory studies.The aim of this study was to investigate the correlation and possible mechanisms of serum SNCA levels and microglia-mediated neuroinflammatory responses measured via ? tumor necrosis factor(TNF-?)and interleukin 6(IL-6)in patients with Parkinson's disease.Methods:20 PD patients who were diagnosed as early and mid-late stages of Hoehn-Yahr classification in a third-tier A hospital were selected as PD group and they were evaluated by UPDRS III and MMSE.At the same time,20 patients in the physical examination center were selected as the healthy control group(HC group).10 ml fasting venous blood in the two groups were collected and centrifuged for supernatant,the serum SNCA,TNF-? and IL-6 were detected by ELISA,and the experimental data were processed by SPSS 24.Results:1.The serum levels of SNCA,?-TNF and IL-6 in the PD group were significantly higher than those in the control group(P<0.01,P<0.01,P<0.05).2.There was a significant positive correlation between SNCA and TNF-? in the PD group(r=0.578;P<0.05).There was no significant correlation between SNCA,TNF-? and IL-6.3.In both groups,serum SNCA,TNF-? and IL-6 levels were not significantly correlated with disease duration,severity of disease,or other disease factors such as blood uric acid levels,hypertension,etc.But IL-6 may be correlated with the severity of disease(P=0.073).4.Between the early and mid-late stages of disease,the levels of IL-6 showed significant difference(P<0.05),but there was no significant difference in SNCA and TNF-? levels between early and mid-late stages.Conclusion:There is a significant positive correlation between serum levels of SNCA and TNF-? in patients with Parkinson's disease,suggesting that there is an intrinsic relationship between serum SNCA and microglial hyperactivation-mediated central nervous system inflammatory responses in the possible pathogenesis of Parkinson's disease,which can affect each other.It has a reference value for the development of later research.