The Investigation Of Biliary Atresia Induced By Interaction Of Add3 Gene Silencing And Rotavirus Infection

Objective:To investigate the mechanism of add3 inducing BA in mouse.Methods: SPF-level Balb/c mice were raised and propagated,and the incident of vaginal plug in female mice was recorded.The experimental group was injected with add3-si RNA via tail vein at 12.5 days of pregnancy.Neonatal mice mice were randomly sacrificed,and the liver tissues of fetal mice were used to detect gene silencing effect.Neonatal mice were induced by intraperitoneal injection of RRV to induce BA.The liver tissues in 7,14,and 21-day-mouse were stained with HE and masson.The expressions of ?-SMA,perforin and TNF-? were compared with the control group by western blot.In vitro experiment was made to explore the mechanism.After extracting the mouse biliary epithelial cells,the add3 gene was silenced,and the cells were infected with RRV,then co-cultured with the mouse NK cells.And NK cell activation markers were detected and compared with the control group.Conclusion: RRV induced add3 gene silencing in mice with heavier liver fibrosis than non-gene silencing mice in vivo.After silencing of the add3 gene,NK cells reduced the amount of active substance secreted after RRV infection both in vivo and in vitro.add3 may be involved in the NK cell activation process,resulting in the occurrence of BA through interaction with NK cells.