Expression And Significance Of ELK1, EPHA7 And RND3 Genes In Nonfunctional Pituitary Adenomas

Objectives:To investigate the relationship and clinical significance between ELK1,EPHA7,RND3 and the invasiveness of non-functional pituitary adenomas,in the hope of providing help for treatment,prognosis and recurrence.Methods:In this study,30 cases of non-functional pituitary adenomas(15 cases of invasive growth,15 cases of non-invasive growth)were selected.By real-time quantitative PCR detection ELK1,EPHA7,RND3 in invasive non-functional pituitary adenomas and noninvasive non-functional pituitary adenomas gene expression differences of two groups of samples between the two,by immunohistochemical method to detect ELK1,EPHA7,RND3 protein in the absence of non-functional pituitary adenomas.Results:Real-time quantitative PCR results showed that the mRNA expression levels of ELK1 and EPHA7 genes in the invasive non-functional pituitary adenoma tissues were higher than those in the non-invasive non-functional pituitary adenoma tissues,while the m RNA expression levels of RND3 genes were significantly lower than those in the non-invasive non-functional pituitary adenoma tissues.Immunohistochemical results showed that ELK1 was strongly positive in the invasive growth group,moderately expressed in the non-invasive growth group,and weakly positive in the normal pituitary tissue.EPHA7 protein is highly expressed in invasive non-functional pituitary adenoma tissues,while weakly positive in non-invasive non-functional pituitary adenoma and normal pituitary tissue samples.RND3 protein was highly expressed in non-invasive non-functional pituitary adenoma specimens,but weakly positive in invasive pituitary adenoma and normal pituitary tissue samples.