| Objective :Whether hematological imbalance in early life affects iron metabolism in adult rats through hematology and iron metabolism;To investigate the effects of iron imbalance on lipid metabolism and oxidative stress in adult rats during early life.Methods : Twelve healthy Wistar female rats with similar body weight were selected and randomly divided into 3 groups after 1 week of adaptive feeding.Low iron group,iron-rich group and control group,4 in each group.Three groups were fed with low-irondiet,iron-rich diet,and common diet,After 2 weeks of feed intervention,Detection of hematological indicators to determine differences between groups,and then mated with healthy Wistar males.The mother continued the feed intervention during pregnancy until the mice were 4 weeks old at the time of birth,during which the maternal and the survival of the female rats were observed and the body weight,hematology and iron metabolism were measured.After the end of the expectation,the iron balance period(ordinary feed feeding)was transferred until the rats were born for 12 weeks.During the balance period,the body weight,hematological parameters,iron metabolism,glycolipid metabolism,and oxidative stress levels were monitored dynamically.To observethe histopathological changes of the liver and cardiovascular system of the rats and the expression of DMT1 in the small intestine of each group.Results :1.Early establishment of iron imbalance model in early life: After 4 weeks of iron intervention in the rats,the body weight and ferritin of the low-iron group were lower than those of the control group(P<0.01),and the weight and ferritin of the iron-rich group were higher than those of the control group(P<0.01);low-iron group RBC,HB,HCT,MCV,MCH,MCHC were lower than the control group,RDW was higher than the control group(P<0.01),HB and HCT in the iron-rich group were higher than the control group(P< 0.01).2.After the iron balance period,the body weight of the low-iron group rats in adulthood was not different from that of the control group(P>0.05),and the body weight of adult rats in the iron-rich group was lower than that of the control group(P<0.05).3.There was no significant difference in hematological indexes between adult low iron group and control group(P > 0.05),but the HB of adult iron rich group was lower than that of control group(P < 0.05).4.There was no difference between the adult low-iron group and the iron-rich group SI and STFR compared with the control group(P>0.05).The Fn in the low iron group was higher than that in the control group(P < 0.01),and the Fn in the iron rich group was lower than that in the control group(P < 0.01).5.In the low-iron group,the GLU,TG,TC,LDL and FFA were higher in the adult group than in the control group(P<0.01),and the HDL was lower than the control group(P<0.01).6.The MDA of adult iron rats was lower than that of the control group(P<0.05),AOPP was higher than the control group(P<0.01),SOD,T-AOC and GST were lower than the control group(P<0.01).MDA in the iron-rich group was higher than that in the control group,SOD,T-AOC,and GST were lower than those in the control group(P<0.01).There was no significant difference between the AOPP and the control group(P>0.05).7.In the adult low-iron group and the iron-rich group,the liver,heart tissue and aorta showed different degrees of steatosis,and lipid droplets and foam cells wereobserved.8.There was no significant difference in the expression of DMT1 gene in the small intestine of each group(P>0.05).Conclusion : 1.Iron deficiency in early life leads to iron load in adult rats,Early iron loading can lead to a decrease in iron absorption capacity in adult rats.Unbalanced iron metabolism in early life can induce oxidative stress in adulthood.Iron deficiency in early life can cause iron load in adulthood,elevated plasma ferritin,and iron ion-induced oxidative stress damage,which leads to disorder of lipid metabolism,which eventually leads to cardiovascular disease. |
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