A Study About Polymorphism Of MTHFRC677T Gene In Patients With Nonneoplastic Epithelial Disorders Of Vulva(NNEDV)

Objective:To investigate the relationship between the polymorphism of MTHFRC677T gene and nonneoplastic epithelial disorders of vulva(NNEDV),and its possible mechanism.Methods:A retrospective analysis of 70 patients with pathologically confirmed NNEDV(Study Group)and 58 healthy women(Control Group)in our hospital between August 2016 to March 2019.We analyzed the difference of the polymorphism of MTHFRC677T gene in two groups.The polymorphism of MTHFRC677T gene,the serum folic acid and serum homocysteine has been compared between different histopathologic types of NNEDV.The study group included 26 cases of vulvar lichen simplex chronicus(LSC)and 44 cases of vulvar lichen sclerosus(LS).Results:A statistically significant increase in the frequency of the MTHFR677TT genotype was found in Study group compared to Control group(14.3%VS 5.2%,χ~2=7.020,P=0.030),and the T allele frequency was notably higher as well(37.1%VS 21.6%,χ~2=7.332,P=0.007).Compared with Control group,the frequency of MTHFR 677 TT genotype(26.9%)and the frequency of T allele(44.2%)in patients with LSC increase significantly(χ~2=8.980,P=0.011;χ~2=9.049,P=0.003).And there is no statistically significant increase in the frequency of the MTHFR677TT genotype(6.8%)and the frequency of T allele(33.0%)in patients with LS(P>0.05).Compared with CC genotype,CT and TT genotype may increase the risk of NNEDV,and the OR is 2.165(95%CI=1.020~4.595)and 4.286(95%CI=1.077~17.061).Serum folic acid level in LS was significantly lower than that in LSC(t=-2.112,P=0.039).There is no significant differences in homocysteine between the patients with LSC and LS(P>0.05).However with the increase of mutant gene T in MTHFR677,the serum folic acid level decreased gradually(F=5.397,P=0.007),and the serum homocyteine level showed a gradual increase trend(F=4.257,P=0.020).Conclusion:Serum folic acid and homocysteine dysmetabolism which induced by mutation of MTHFRC677T gene may be associated with LSC.LS is not related to the mutation of MTHFRC677T,but it may be related to folate deficiency and homocysteine accumulation induced by other reasons.