| Objective:Psoriasis is a common chronic inflammatory skin disease and the pathogenesis of psoriasis is unknown.It is generally believed that its pathogenesis is related to nerves,environment,heredity,infection,metabolism and immunity.The neuroendocrine system plays an important role in the inflammatory response of psoriasis,in which neuropeptides play an indispensable role.The purpose of this study was to investigate the correlation between neuropeptide CGRP1/VIP and the severity of psoriasis.Methods:1.Psoriasis patients admitted to the affiliated hospital of chengde medical college were selected.The levels of neuropeptide CGRP1 and VIP in the psoriasis patients were detected by enzyme-linked immunosorbent assay(ELISA).2.According to the psoriasis lesion area and severity index(PASI)score,patients were divided into four groups:mild,moderate and severe psoriasis vulgaris and control group,with 20 cases in each group.The plasma levels of neuropeptides CGRP1 and VIP were observed.3.After routine treatment of psoriasis vulgaris,40 patients with psoriasis who were effectively treated were selected to observe and compare the changes of CGRP1 and VIP in before and after 14 days of treatment.4.The levels of CGRP1 and VIP in psoriasis vulgaris,erythrodermic psoriasis,pustular psoriasis and joint psoriasis were observed.5.To observe the correlation between the severity of psoriasis vulcanis and clinical indicators such as blood routine,ion,blood lipid,liver function and kidney function.6.Statistical software SPSS 22.0 was used for statistical analysis of the data.Count data was using?~2 inspection.Comparison of measurement data between the two groups showed that variance homogeneity in line with normal distribution was tested by independent sample t test,variance heterogeneity was tested by independent sample correction t test,and paired sample t test was used in before and after treatment.The data results were measured by mean standard deviation(?).The rank sum test was applied to the measurement data that did not conform to the normal distribution,and the results were expressed as M(P25,P75).Compared among the three groups,the measurement data were in line with the normal distribution were analyzed by the single-factor method,,and the data that did not conform to the normal distribution were analyzed by the kruskal-wallis H rank sum test.P<0.05 was considered statistically significant.Results:1.Gender and age in mild,moderate and severe of psoriasis vulgaris group and blank group were statistically analyzed,and the difference was not statistically significant(P>0.05).2.The mild,moderate and severe psoriasis vulgaris group had higher CGRP1 and VIP level than the blank group,and the difference was statistically significant(P<0.05).3.The mild psoriasis group had lower CGRP1 and VIP level than the moderate and severe group,and the difference was statistically significant(P<0.05).The moderate psoriasis group had lower CGRP1 than the severe psoriasis group,and the difference was statistically significant(P<0.05).There was no significant difference in VIP between the moderate psoriasis group and the severe psoriasis group(P>0.05).4.There was no significant difference between the psoriasis vulgaris group and erythrodermic psoriasis group and pustular psoriasis group(P>0.05).In this study,a total of 2 cases of articular psoriasis were collected,with CGRP1 of 0.86,0.42 ng/ml,and VIP of 3.50 and 9.36 ng/ml.5.CGRP1 after treatment level was lower than before treatment,and the difference was statistically significant(P<0.05).6.Single factor analysis was used to analyze the influence of clinical indicators with the severity of psoriasis vulgaris,and the difference was not statistically significant(P>0.05).Conclusion:The plasma levels of CGRP1 and VIP in patients with psoriasis were higher than those in the blank group,which may be related to the severity of psoriasis... |
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