Study On The Effect And Mechanism Of Angelica Sinensis In Xinwen Jiebiao And Huoxue Zhitong

Objective To evaluate the effect of angelica sinensis on hyperthermia,blood circulation,blood stasis and pain,and elaborate its related mechanism of action,providing a solid experimental and theoretical basis for its clinical application and development.Methods The acute toxic effects of volatile oil of Angelica sinensis on mice were explored.Then the antipyretic effect of Angelica sinensis volatile oil was evaluated in fever rats induced by yeast,LPS or 2,4-dinitrophenol,the analgesic effect was measured by acetic acid writhing method and hot plate in mice,the anti-inflammatory effect was evaluated by xylene-induced ear swelling in mice or carrageenan-induced foot swelling in rats.The distribution of volatile oil of Angelica sinensis in rats was investigated.The effects of volatile oil of Angelica sinensis on the activities of monoamine oxidases(MAOs),cyclooxygenases(COXs)and 5-lipoxygenase(5-LOX)were studied to explore the possible action mechanism of the volatile oil of Angelica sinensis.Results The results showed that the volatile oil of Angelica sinensis has some acute toxicity,after treatment,the mice moveed slowly,prone or no movement,breath shortly,blepharochalasis,and individual body weakness,limbs and body trembling.There was no change in organs in the dead mouse.The LD50 was 1682.6 mg/kg.The volatile oil of Angelica sinensis 300 mg/kg had good antipyretic effect on fever induced by yeast,LPS or 2,4-dinitrophenol in rats,significantly increased the pain threshold of hot plate method in mice;decreased the number of writhing induced by glacial acetic acid in mice in a dose-dependent manner;and inhibited the foot swelling induced by carrageenan in rats.And the volatile oil of Angelica sinensis was found to suppress uppress ear swell induced by xylene in mice.Pharmacokinetic study showed that at 0.5,1,2,3,4 hours,ligustilide was detected in plasma and cortex after the volatile oil from Angelica Sinensis were adiministered by intragastric administration,and the highest content was observed at 1 h.Enzyme kinetics study found that the IC50 of Angelicae sinensis volatile oil for MAO-A was 37.71 ?g/ml,for MAO-B was 62.79 ?g/ml.Angelica sinensis volatile oil is a reversible inhibitor of MAO-A and MAO-B;Angelica sinensis volatile oil can competitively inhibit both MAO isozymes.The IC50 of volatile oil of Angelica sinensis was 22.27 ?g/ml for COX-1,4.26 ?g/ml for COX-2 and 94.48 ?g/ml for 5-LOX.Conclusion The volatile oil of Angelica sinensis has good antipyretic,analgesic and anti-inflammatory effects,which are closely related to its traditional efficacy.Its mechanism is partly related to its ability to inhibit the activity of monoamine oxidases(MAOs),cyclooxygenases(COXs)and 5-lipoxygenase(5-LOX).