| Objective: To observe the changes of intestinal microorganisms in T2 DM patients,to study the effect of ShenQi FuFang on the intestinal tract of T2 DM patients,and to explore the mechanism of traditional Chinese medicine affecting intestinal microorganisms,so as to provide theoretical basis for the prevention and treatment mechanism of traditional Chinese medicine compound preparation and the development of new drugs.Methods: The newly diagnosed T2 DM patients in the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine were collected and included into the subjects according to the study requirements.The subjects were divided into the ShenQi FuFang group(experimental group),lifestyle intervention group(control group)and healthy people group(blank control group)using the random number table method.The experimental group was given ShenQi FuFang prescription(exempted from inspection granule preparation)combined with lifestyle intervention,taking method: 1 dose a day,1 grid a time,3 times a day,100 ml warm water infusion,30 minutes before meals.The control group received only lifestyle intervention.Observation and follow-up were conducted for 4 weeks,and basic information of the patients was recorded weekly.Observe before and after treatment of two groups of subjects to fasting plasma glucose,gut microbes,postprandial 2 hours blood sugar,TCM syndrome integral,glycosylated hemoglobin,safety as well as blood routine and biochemical indexes,and the gap with blank control group,and evaluate the comprehensive curative effect,using the second generation of high-through to put sequencing technologies acer for sequencing and objectively reflect the real changes of microorganisms.SPSS22.0 statistical software was used for statistical analysis of all data.Results :(1)After statistical analysis there is a comparative study value in the two groups of subjects in the course of disease,TCM syndrome points and degree of severity and general basic conditions,etc.,before treatment.(2)Condition comparison: Both the experimental group and the control group were able to improve fasting blood glucose,glycosylated hemoglobin and2-hour postprandial blood glucose(p<0.05),and the experimental group had better regulation of fasting blood glucose,glycosylated hemoglobin and 2-hour postprandial blood glucose in T2 DM patients(p<0.05).(3)Syndrome changes: The experimental group and the control group showed significant improvement in symptoms of T2 DM patients,such as dryness of pharynx and mouth,tiredness and fatigue,abdominal distension,defecation,urination,heavy body sleepiness and hand-foot-heart heat(p<0.05).The experimental group showed better improvement in symptoms of T2 DM,such as dryness of pharynx and mouth,tiredness and fatigue,limb numbness/pain and hand-foot-heart heat(p<0.05).(4)Intestinal microorganisms: Bacteroides increased in the experimental group compared with the control group,while actinomycetes and klebsiella decreased compared with the control group(p<0.05).Compared with the healthy people group,streptococcus,Coprococcus and enterococcus were increased in T2 DM patients(p<0.05).Conclusion:(1)The study found that type 2 diabetes mellitus patients are intestinal microecological disturbance.(2)Streptococcus,Coprococcus and enterococcus may play a role in the pathogenesis of diabetes?(3)ShenQi FuFang can improve blood glucose metabolism,TCM symptoms and intestinal microbial composition in patients with type 2 diabetes mellitus.The mechanism of ShenQi FuFang to improve blood glucose metabolism,TCM symptoms in type 2 diabetes mellitus patients may be related to the effect of intestinal microorganism and the improvement of metabolism. |
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