| Objective:Neonatal high unconjugated bilirubinemia is a common disease in neonates,characterized by clinical manifestations of yellowing of skin sclera and elevated serum unconjugated bilirubin.Phototherapy is a simple and easy way to reduce serum stagnation and bilirubin.Various phototherapy devices using different light sources can be used for phototherapy.Neonatal antioxidant resistance is limited,and recent studies have shown that phototherapy affects the oxidative/antioxidant state.Our aim was to investigate the effects of phototherapy with different light sources on neonatal oxidation/antioxidant balance after different phototherapy times.Methods: Enrollment-50 full-term newborns(37 weeks to 42 weeks)who were admitted to our hospital for phototherapy mainly from May 2018 to December without combined bilirubin hyperplasia were randomly divided into two groups.Infants using traditional fluorescent lamps were defined as group 1,and those using intensive light emitting diode(LED)were defined as group 2.Analyze serum levels of 8-hydroxy-2-deoxy-guanosine(8-OHdG),malondia-ldehyde(MDA)and superoxide dismutase(SOD)before phototherapy,24 hours,48 hours and 72 hours after light therapy.Results: Compared with the LED phototherapy group,the traditional phototherapy group:1.After 24 hours of phototherapy(t=0.586,P=0.563),48 hours after phototherapy(t=0.238,P=0.814),and 72 hours after phototherapy(t=1.871,P=0.074),there was no significant difference in serum 8-ohdg level between the two groups.2.After 24 hours of phototherapy(t=2.029,P=0.055),48 hours after phototherapy(t=0.895,P=0.380),and 72 hours after phototherapy(t=0.522,P=0.607),there was no statistically significant differences in serum MDA levels between the two groups.3.After 24 hours of phototherapy(t=1.075,P=0.293)and 48 hours of phototherapy(t=0.421,P=0.677),there was no significant difference in serum SOD level between the two groups.After 72 hours of phototherapy,the serum SOD level in the traditional phototherapy group decreased,while that in the LED phototherapy group did not change significantly(t=2.170,P=0.040).Comparison in terms of different phototherapy time:1.8-OHdG level:1)Traditional phototherapy group: After 24 hours of phototherapy,after 48 hours of phototherapy,there was no significant difference in serum 8-OHdG level and phototherapy before phototherapy for 72 hours(P value: 0.222,respectively).0.129,0.873);2)LED phototherapy group: After 24 hours of phototherapy,after 48 hours of phototherapy,there was no significant difference in serum 8-OHdG level after phototherapy for 72 hours(P value: 0.738,0.387,0.565).2.MDA level: 1)traditional phototherapy group: serum MDA level decreased after phototherapy and phototherapy,serum MDA level after 24 hours of phototherapy was not statistically significant(P=0.207),serum MDA after 48 hours of phototherapy The difference between the level and the phototherapy was statistically significant(P=0.041).The serum MDA level after 72 hours of phototherapy was significantly different from that before phototherapy(P=0.003).2)LED phototherapy group: the serum MDA before phototherapy declined compared with after phototherapy and each time were significantly different from those before phototherapy(p values: 0.011,0.044,0.012).3.SOD level: 1)Traditional phototherapy group: The level of serum SOD decreased after phototherapy and before phototherapy,but after 24 hours of phototherapy,the difference of serum MDA level after phototherapy 48 hours was not statistically significant(p value was respectively 0.286,0.588),the serum MDA level after phototherapy 72 hours was significantly different from that before phototherapy(P=0.025);2)LED phototherapy group: 24 hours after phototherapy,48 hours after phototherapy,72 hours after phototherapy,serum SOD level and before phototherapy There was no statistically significant difference(P-value: 0.166,0.677,0.921).Conclusion: No traditional phototherapy or LED phototherapy has caused damage to the body's DNA.With the increase of phototherapy time,traditional phototherapy and LED phototherapy can reduce oxidative stress damage.The difference between the two is small,but the MDA level after phototherapy is significantly reduced.It is due to the decrease of serum bilirubin level or the influence of phototherapy itself,which needs further research and determination;LED phototherapy has no effect on the body's antioxidant capacity,but with the increase of phototherapy time,traditional phototherapy can reduce the antioxidant capacity. |
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