The Implant Bio Compatibility Of Ultrasonic Micro-arc Oxidation Microarc Oxidation,MAO BLF Compound Coating

Purpose: In this experiment,after the treatment of pure magnesium matrix by ultrasonic micro-arc oxidation(MAO),Silane-coupled bovine lactoferrin as a "molecular bridge" using silane as a coupling agent to form a composite membrane implant of Mg-MAO-bovine lactoferrin,To investigate the effect of pure magnesium ultrasonic micro-arc oxidation layer on the healing ability of bovine lactoferrin.Methods: Nine rabbits were divided into three groups,with pure magnesium ultrasonic micro-arc oxidationas the control group(a).The experimental group was pure magnesium micro-arc oxidation-silane(b)group,pure magnesium micro-arc oxidation-silane loading,Bovine lactoferrin(c)group.Two magnesium substrates were implanted into the mandible of the same side of the rabbit,and the mandibular tissue containing the magnesium matrix was taken out at 2,4 and 8 weeks after implantation.n the absence of decalcification,Cone beam computed tomography(Cone beam CT,CBCT)was used to observe the binding and degradation of magnesium matrix and bone tissue;Scanning electron microscopy was used to observe the binding of bone interface,and the spectrum analyzer was used to determine the distribution of surface elements of different coatings;Laser confocal detection was used to judge the growth of new bone by the intensity of the fluorescent deposition zone..Results: The bone tissue healing was observed by CBCT.The sagittal bone tissue healed well in the 8th week(c)group,and there was less uniform density of new bone tissue around it,There is also a certain degree of bone tissue healing in groups(a)and(b);Scanning electron microscopy and energy spectrum analysis showed that the bone tissue interface between the 8th and 8th groups was more closely related to the around the magnesium nail.There is a large amount of calcium and phosphorus deposits between the magnesium matrix and the bone tissue;Laser confocal display showed that the 8th(c)group of fluorescent deposition zone had the best intensity and the widest width,and the measurement and calculation of bone deposition rate showed that,in the comparison between groups,the value of group 8(c)was 1.71 ± 0.15,which was larger than 1.65 ± 0.25 in group 2(a)and 1.62 ± 0.16 in group 4(c),In the intra-group comparison,the values of the 8-week(c)group were 1.71±0.15,which were larger than the 8-week(a)group 0.93±0.067,and the 8-week(b)group 1.101±0.13,it is proved that the layer treated with bovine milk protein after micro-arc oxidation silane treatment has better biocompatibility.The degradation rate is slower and the bone tissue is more generated.Conclusion: Pure magnesium ultrasonic micro-arc oxidation uses silane as a coupling layer of 0.1 ?g/L bovine lactoferrin composite membrane,which makes the magnesium matrix more capable of promoting bone healing,enhance biocompatibility,shorten the absorption time of magnesium matrix,and provide a new method for clinical treatment of bone defects.