Clinical Information Analysis And Genome-wide DNA Methylation Of Young Hepatocellular Carcinoma Patients In Southern Fujian

Primary liver carcinoma(PLC)is one of the most common malignant tumors in the world,of which hepatocellular carcinoma(HCC)accounts for more than 80%.New cases and deaths of liver cancer occur in China each year,accounting for more than 50% of the world.Fujian is one of the high incidence areas of liver cancer in China.According to reports,the incidence of malignant tumors in Fujian Province in 2014 was 269.12/100,000,and that of liver cancer was the third;the mortality rate of malignant tumors was 164.40/100,000,and liver cancer ranked first for nearly 20 years.It is found that the age of onset of liver cancer in China is mainly concentrated in 40-60 years old,and the proportion of patients under 40 years old is less than 10%.However,in recent years,the incidence of liver cancer under 40 years old has increased at a rate of 1%-2% per year,causing Social attention.This article based on whole-genome methylation sequencing technology aims to investigate the differences in the mechanisms of liver cancer in young liver cancer patients(?40 years old)and middle-aged and elderly people(>40 years old).The research contents:(1)retrospectively analyze the clinical data of hospitalized HCC patients in Longnan area,and divide them into young HCC group and middleaged HCC group to study the epidemiological characteristics of young HCC patients in Longnan area.(2)Using genome-wide DNA methylation sequencing technology to construct a genome-wide DNA methylation expression profile of whole-genome range in young HCC patients and middle-aged HCC patients,in order to study the occurrence of young HCC and middle-aged HCC patients The developmental differential mechanism provides a theoretical basis for GO and KEGG pathway enrichment analysis of differentially methylated genes and screening for key genes.(3)Analyze the expression of key genes using an online database.Results:(1)The results of this study found that young HCC patients mostly occurred in rural men.Most of them were diagnosed with advanced liver cancer,the rate of surgical treatment was low,and the liver function classification was mostly grade B and C.The HBV infection rate and ALT of young HCC patients were confirmed.The positive rates of AST and AFP were significantly higher than those of middle-aged and elderly patients(p<0.05).(2)The analysis found that there were 11993 DMRs in the young HCC group and the middle-aged HCC group,corresponding to 4260 DMGs.The sites with significant differences are mostly distributed in the CHG and CHH sequence environments.The GO function annotation and KEEG pathway enrichment of DMGs were found to be significantly enriched in biological processes related to tumor development,such as bioadhesion and cell development.Significant enrichment pathways in the promoter region include ribosomes,phagocytosis and other pathways.The genes involved in the significant enrichment pathway were analyzed,and many genes that have been reported to be associated with tumors,such as RPL11 and MMP14,were reproduced,and the key gene TAP2 for the developmental differences between young HCC patients and middle-aged HCC patients was unearthed.(3)The methylation level of the promoter region of TAP2 was significantly lower in the young HCC group than in the middle-aged HCC group.Through UALCAN database analysis,it was found that the methylation level of TAP2 promoter region in HCC tissues was significantly lower than that in normal liver tissues,and it was found to be highly expressed in HCC tissues by GEPIA analysis.Conclusion: This study is the first to explore the epidemiological characteristics of HCC in young people in southern Fujian.Whole-genome DNA methylation sequencing revealed differences in methylation in young HCC patients and middleaged HCC patients,and screened for the key gene TAP2,whose promoter region was significantly lower in young HCC than in middle-aged HCC.Group,and its expression level is negatively correlated with the methylation level of the promoter region,and the high expression of TAP2 may be used as a detection index for young HCC.