Detection Of Differential Genome-wide DNA Methylation In Peripheral Blood Of Asthmatic Patients And Its Clinical Significance

Background Bronchial asthma,a common chronic inflammatory disease of the respiratory tract,is a type I allergic disease.In recent years,the incidence of asthma has gradually increased,and research on the pathogenesis of asthma has gradually become a hot spot.Epigenetics is a genetic inheritance of nucleic acids that is not based on DNA differences.Under the premise that the DNA sequence is not changed during cell division,all cells undergo meiotic or mitotic heritable changes in phenotype or gene expression.Because human genetic background is unlikely to change significantly in the short term,the role of epigenetic modification in regulating environmental and genetic factors is particularly important.Current research results show that complex genes and environment interactions are jointly involved in the pathogenesis of asthma,which may be the result of epigenetic changes in patients after successive exposure to the environment,such as DNA methylation modification,histone covalent modification,mi RNA Changes,such as changes in chromatin,etc.,for epigenetic treatments may open up new ways for individualized and accurate treatment of asthma.This study examined clinically collected peripheral blood genomic DNA methylation in patients with acute asthma and healthy controls,and analyzed the epigenetic differences in DNA methylation between patients with acute asthma and healthy controls for screening.The key genes and signal pathways in the development and development of asthma are provided to provide new targets and strategies for asthma treatment.Objective To investigate the role of DNA methylation in the pathogenesis of asthma,and tofind new targets for the diagnosis and treatment of asthma.Methods Selecting three adult patients with healthy diagnosis and three healthy controls.and than the peripheral blood DNA was extracted,the whole genome DNA methylation level was detected using the Illumina DNA methylation chip,and it intersected with the 96 gene chip data samples in the downloaded GSE56553 data set,and biological analysis of differential gene functional pathways was performed.Results Peripheral blood genomic DNA methylation levels of asthmatic patients and healthy controls were significantly different.1493 differential genes were found,of which923 genes were methylated up-regulated,570 genes were methylated reduced,and GO and KEGG pathway functions The differential genes obtained by enrichment analysis are mainly focused on the biological processes involved in the regulation of synaptic signaling,muscle contraction,and cell adhesion.TNF,GNB1,PIK3R1,CDC5 L,IL10,CD44,etc.were screened by constructing gene interaction networks Key genes.Conclusions There is a significant difference between the degree of methylation of genomic DNA in peripheral blood of asthmatic patients and healthy controls,and this difference is likely to be the cause of asthma development.Using bioinformatics methods to screen out key genes and signaling pathways in the development of asthma can provide new targets and strategies for asthma treatment.