Physicochemical Characterization And Biological Effect Of Anorganic Bovine Bone Matrix And Organic-Containing Bovine Bone Matrix In Comparison With Bio-Oss

For serious bone defects,such as after the cyst excision,tumor excision,severe periodontitis bone absorption,additional measures should be taken to increase the long-term implant success rate.The application of bovine bone matrix is a hotspot.However,in the market,bone grafts have a lot of categories,which make clinicians hard to choose a cost-effective and good biological bone graft.Bovine origin matrix has been widely used in clinical applications and investigated by various research institutions.However,potential factors of influencing bone regeneration are still not thoroughly understood and need further investigations.In this study,anorganic bovine bone matrix(ABBM),organic-containing bovine bone matrix(OBBM)and widely acknowledged anorganic bovine bone matrix(Bio-Oss)were selected as experimental materials.Physiochemical characterizations and bone regeneration properties of the three bone matrixes were investigated.Besides,the correlations between physiochemical characterizations and bone regeneration properties were also investigated.Physiochemical characterizations were measured by SEM,XPS,XRD,FI-IR and BET instruments,In vivo animal experiment,8-mm-diameter defects were created in the calvaria of New Zealand White rabbits and filled with the abovementioned bone matrixes.The samples were harvested at 6 and 12 weeks post-surgery,and evaluated by micro-computed tomography(u-CT)and histological analyses.The physical characterizations showed that ABBM and Bio-Oss had more nanostructures,larger surface area,bigger pore volume,and bigger pore size than that of OBBM.The chemical characterizations showed that ABBM and Bio-Oss had higher crystallinity than that of OBBM,and OBBM contained organic nitrogen(N)component.In vivo,ABBM and Bio-Oss possessed better bone regeneration properties than that of OBBM.Taken together,nanostructures,larger surface area,bigger pore volume and bigger pore size of xenografts played an active role in new bone formation.Besides,lower crystallinity and organic N element of xenografts produced a positive effect on graft degradation.This study explored the characterizations and biological effects of three bovine bone matrixes.It was found that the biological effects of ABBM and Bio-Oss are better,and some correlations between characterizations and biological effects of bovine bone matrixes.The abovementioned findings could provide a theoretical basis for better choice in clinical applications and better manufacturing HA-derived bone graft in the future.