The Mechanism Of LncRNA SNHG1 Promoting The Proliferation Of Gastric Cancer Cells Via Degrade MiR-145/MYC

Background and ObjectsThe mortality rate of gastric cancer ranks second in malignant tumors worldwide,and China is a country with high incidence of gastric cancer.The newly discovered IncRNA small nucleolar RNA host gene 1(SNHG1)has a potential binding site with miR-145,and the expression levels of the two are negatively correlated in various tumors.Based on the above results,we believed that SNHG1 plays an important role in promoting the growth of gastric cancer cells,and speculate that SNHG1 can act as a miR-145 ceRNA,further affecting the regulation of miR-145 on downstream c-Myc.MethodsThe expression of SNHG1 in gastric cancer tissues was detected by real-time PCR.The effect of SNHG1 on the proliferation of gastric cancer cells was detected by CCK8.The correlation between the two expressions in gastric cancer tissues was verified by online database bioinformatics analysis.Binding site.The relationship between the two was verified by qRT-PCR,and the direct binding effect of SNHG1 on miR-145 was verified by the dual luciferase reporter gene system.The effect of both miR-145 or(and)SNHG1 on the expression of CTNNB1 and MYC was verified by western blot.The experimental data were analyzed by software SPSS 21.0.The pairwise comparison between groups was performed by paired sample t-test test,and the non-parametric test was used for non-parametric test.P<0.05 indicated that the difference was statistically significant.ResultsThe expression of SNHG1 in 17 pairs of gastric cancer tissues was significantly higher than that in adjacent tissues(p<0.0001),which was consistent with the data analyzed by starBase v3.0 database(p=1.4-1014,FDR=4.3×1013).CCK-8 detection found that SNHG1 can promote the proliferation of gastric cancer cells.Combined with TANRIC database,starBase v3.0 database,qRT-PCR detection and luciferase reporter gene assay,SNHG1 can negatively regulate the expression of miR-145.Western blot analysis revealed that miR-145 and SNHG1 had a certain effect on the CTNNB1/MYC pathway.The Kaplan-Meier Plotter database was used to analyze the overall survival of patients with gastric cancer and the survival after surgery.The survival of the high expression group of SNHG1 was significantly lower than that of the low expression group(p=0.015;p=0.0061).ConclusionSNHG1 promotes the proliferation of gastric cancer cells by competitively binding to miR-145,thereby increasing the expression of the downstream target gene MYC.