| ObjectiveTo detect the expression of long chain non coding RNA(Homo sapiens testicular cell adhesion molecule 1 homolog)in gastric cancer tissues and cells,and to observe the effect on the proliferation of gastric cancer cell line.BackgroundGastric cancer is one of the most common malignant tumor threaten human health,although with the improvement of medical standards,the diagnosis and treatment of gastric cancer have made great progress,but the incidence rate and the mortality rate remains high.Therefore,to carry out basic research to explore the incidence of gastric cancer,gastric cancer,development mechanism,so as to prevent the early diagnosis of gastric cancer.Is of great significance to provide reliable support.The tumor occurrence,development is the result of various factors,and in different stages of tumor development,the same factor may plays a different role.The environmental risk factors and is closely related to the occurrence of tumor,can cause abnormal expression of genes,thus cause the corresponding biological function change in common effect of gene environment interaction,eventually causing cancer.Long chain encoding RNA(Long non-coding non RNA,IncRNA)in the tumor to the shift key control Has aroused people’s attention by.LncRNA is an important gene transcription regulatory elements.through epigenetic,transcriptional and post transcriptional regulation and other aspects of the regulation of gene expression.A large number of studies have shown that there are abnormal expression of lncRNA molecules in many cancers,such as prostate cancer,bladder cancer,breast cancer.MethodA total of 122 patients with gastric cancer treated at the Department of general surgery in Jingzhou central hospital were entered our researchment.Specimens of gastric cancer tissues and tissues adjacent to the cancer tissues were collected.All specimens were proved to be gastric cancer by H.E staining,then stored in liquid nitrogen tank.The pathological specimens were selected for gene chip detection,and the difference of gene expression between cancer tissues and paracancerous tissues was analyzed.The expression of TCAM1 in gastric cancer,paracancerous tissues,gastric cancer cell lines and normal gastric mucosa cell lines was detected by qPCR in vitro.Transfect the siRNA targeting TCAM1 into gastric cancer cells,and construction a cell lines stably expressing LNC RNA TCAM1.The proliferation,migration and invasion of the stable cells were detected,and the cell cycle was detected by flow cytometry.To evaluate the function and mechanism of lncRNA TCAM1 in the carcinogenesis and development of gastric carcinoma.ResultBased on the results of previous lncRNA microarray screening,we chose the lnc-TCAM1 with the largest multiple difference in the two sets of data as the object of the next study.QPCR was used to detect the expression of TCAM1 and mRNA in 122 pairs of gastric cancer and its adjacent tissues,and GAPDH was used as an internal control.By comparing the CT values,it was found that TCAM1 and mRNA showed an upward trend in most tumors.In the cell line,we also found that TCAMI was highly expressed in gastric cancer cell lines compared with immortalized gastric mucosal cell GES1.The expression of TCAM1 is related to clinicopathological features.The expression was elevated in the low differentiation,the deeper invasion and the larger tumor group,and the difference was statistically significant(P<0.05).The plasmid over expressing TCAM1 was transfected into gastric cancer cell SGC7901,TCAM1 up-regulated the expression of 50%.TCAM1 expression was up-regulated by bout 50%.After that,the proliferation ability of SGC7901 cells and TCAM1 overexpressing SGC7901 cells was detected,and the overexpression of TCAM1 significantly increased the proliferation of gastric cancer cell line SGC7901.The cell cycle was detected by flow cytometry.We found that overexpression of TCAM1 increased the percentage of G2/M cells.According to the observed results can be seen after significantly enhanced expression of TCAMI cell migration,invasion ability;according to the cell counting results,expressionSGC7901 cell number was significantly higher than that of TCAM1 cells transfected with empty plasmid,significant difference(P<0.05).After transfection with TCAM1-siRNA in gastric cancer cell line SGC7901,we found that the inhibitory rate of TCAMI expression was>90%.After that,we examined the cell proliferation ability of the experimental and control groups.We found that knockdown of TCAMI significantly inhibited the proliferation of gastric cancer cell line SGC7901.The cell cycle was detected by flow cytometry,and it was found that knockdown of TCAM1could reduce the number of G2/M cellsConclusion1 The expression level of lncRNA and TCAM1 in gastric cancer tissue increased significantly,and there was a significant correlation between lymph node metastasis and gastric cancer TNM staging.LncRNA TCAM1 can be used as a biomarker for the occurrence,development and clinical prognosis of gastric cancer,and has good clinical reference value.This study provides an effective way for the role and mechanism of lncRNA and TCAMI in the carcinogenesis and development of gastric carcinoma.2.Lnc TCAM1 can promote the proliferation of gastric cancer cells,enhance their invasion and invasion ability,and have a significant impact on the cell cycle of gastric cancer cells... |
