| Objective:To investigate the effect of folic acid on oxidative damage in serum and organs of rats with acute lead exposure,and according to the expression of Nrf2,HO-1,FTO,METTL3 protein in brain tissue and the change of m6A content in total RNA.To determine whether folic acid has a certain repair effect on oxidative damage induced by lead exposure,to explore whether lead exposure can reduce the content of m6a in brain tissue and whether folic acid has protective effect on it,so as to provide a theoretical basis for the treatment of acute lead poisoning.Methods:40 6-week-old male rats were randomly divided into 4 groups by free drinking water model to establish acute lead exposure model.The control group was group C(deionized water and deionized water intragastrically),and the lead exposed group was group L(0.2%lead acetate solution and deionized water intragastrically).Lead exposure+folic acid treatment group was group T(0.2%lead acetate solution and 0.4mg/kg bw folic acid intragastrically).Folic Acid group F(deionized water and 0.4mg/kg·bw folic acid intragastrically).The total duration of acute lead exposure was 14 days.the lead exposure+folic acid treatment group and folic acid group began intervention with folic acid on the 7th day of lead exposure.After the acute lead exposure model was successfully established,the serum,heart,liver,spleen,lung,kidney,hippocampus and cortex of rats in each group.were taken.Blood centrifugation was used to separate serum and plasma.After the plasma was digested by wet method,the content of lead in blood was determined by graphite furnace atomic absorption spectrometry(GFAAS),and the learning ability of rats in each group was observed by Morris water maze test.Malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,glutathione(GSH)content,Glutathione peroxidase(GSH-PX)activity in serum and various organs were determined by WST-1 method,TBA method,colorimetry and spectrophotometry,respectively.The expression of Nrf2,HO-1,FTO and METTL3 protein in hippocampus and cortex was detected by immunofluorescence,and the content of m6A in hippocampus and cortex was detected by EpiQuikTM m6A RNA methylation quantitative.Results:The content of lead in blood in lead exposure group and folic acid treatment group was significantly higher than that in control group and folic acid group(P<0.01),and in Morris water maze test,the learning ability of rats in folic acid group was significantly higher than that in lead exposure group and control group(P<0.01),and the learning ability in control group was higher than that in lead exposure group(P<0.05);According to the oxidation index,SOD,GSH,GSH-PX decreased significantly and MDA increased significantly in serum,heart,liver,spleen,lung and kidney in lead exposed group compared with the control group(P<0.05).The control group was compared with the folic acid group,in folic acid group,MDA decreased significantly in heart and lung(P<0.05),MDA,SOD,GSH,GSH-PX)in serum,liver,spleen,lung and kidney,but the difference was not statistically significant.The content of GSH in hippocampus and cortex in lead exposure group was significantly lower than that in control group,folic acid group was significantly higher than that in lead exposure group,and folic acid group was higher than that in control group(P<0.05).Immunofluorescence results showed that in histrategies ppocampus and cortex,The average integral optical density(IOD/area,mean density)of Nrf2 and HO-1 immunofluorescence positive reactants in lead exposure group was significantly higher than that in control group,folic acid group was significantly lower than that in lead exposure group,and folic acid group was lower than that in control group.The difference was statistically significant(P<0.01).The expression of METTL3 in lead exposure group was significantly lower than that in control group(P<0.05),and the expression of FTO in lead exposure group was significantly higher than that in control group(P<0.05).The results of EpiQuikTM m6A RNA rhethylation quantitative detection kit showed that the content of m6A in hippocampus and cortex of lead exposed group was significantly higher than that of control group,and that of folic acid group was significantly lower than that of control group(P<0.05).Conclusion:Acute lead exposure can cause oxidative damage in serum,heart,liver,spleen,lung and kidney of rats,reduce learning and memory ability of rats,and reduce the level of RNA m6Ain hippocampus and cortex.Folic acid can effectively eliminate free radicals in the body and inhibit lipid peroxidation.By reducing the production of MDA and increasing the activity of SOD,GSH,GSH-PX,folic acid can repair the oxidative damage caused by acute lead exposure.It also promoted the ability of learning and memory,and increase the level of RNA m6A in hippocampus and cortex. |
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