The Intervention Effect Of Pleurotus Eryngii Polypeptides On Oxidative Damage Of Rats Induced By Lead

[Objective] The experiments mainly investigated the nutrition intervention effect of the Pleurotus eryngii polypeptides on lead induced oxidative damage of viscera organ in rats.[Method] 27 SD male rats aged 21 d were randomly divided into three groups, including control group (basic diet+ distilled water), lead-exposed group (basic diet+ 0.2% lead acetate aqueous solution) and test group (basic diet+ 2% Pleurotus eryngii polypeptides+0.2% lead acetate solution). After two months, the blood of rats were collected. The hearts, livers and kidneys of rats were handled to detecte the contents of Pb?Ca?Fe?Zn?Mg?Cu?Mn by the method of ICP-AES. Then the activities of SOD, GSH-Px, CAT and the level of MDA in serum and various organs were detected by the relevant Kits; Finally, we prepared the paraffin section and HE stain of rat liver tissue to observe the histological changes.[Result] (1) Compared with the control group, there was no significant difference in the liver and kidney index of viscera organ in lead group (P>0.05). The liver and kidney index were no significantly difference in experimental group compared with the lead group (P>0.05).(2) Compared with the control group, the Ca, Fe, Zn, Mg, Cu, Mn content of liver, kidney and heart decreased in lead-exposed group. Among this, the level of Ca, Fe, Zn, Cu in liver, kidney and heart decreased significantly (P<0.05 or P<0.01). The Mg level in liver and heart, and Mn level in kidney also significantly decreased (P<0.05). Compared with the lead exposed group, the Pb content of liver, kidney and heart decreased significantly (P<0.05), however, the content of iron and copper in kidney, the calcium, iron, zinc, copper in liver, and the zinc, magnesium in heart increased significantly (P<0.05 ?, P<0.01).(3) Compared with the control group, the activity of SOD, CAT and GSH-Px in liver and serum, the activity of SOD and GSH-Px in kidney and the activity of SOD and CAT in heart significantly decreased (P<0.05). The levels of MDA extremely significantly increased in lead-exposed group (P<0.01). Compared with the lead-exposed group, the activity of CAT and GSH-Px in liver, SOD and CAT activity in heart, and the SOD, CAT, GSH-Px activity in serum significantly increased (P<0.05 or P<0.01), while the levels of MDA significantly decreased in serum, liver and kidney (P<0.05 or P<0.01).(4) In the control group, we found that there was normal hepatic cell morphology. The hepatic lobule structure was intact, the hepatic sinusoid was clear, the hepatic cord arranged in neat rowsand there was no obvious vacuole. Compared with the control group, the hepatic cells had focally vacuoles, edema, and the liver cords was not clear in lead exposed group. In addition, the glomerular basement membrane was thickened, the mesangial area was widened and it had partial vacuolization, while there was a certain degree of improvement in experimental group.[Conclusion] Pleurotus eryngii polypeptides can reduce the content of lead and regulate the balance of mineral elements in the body. It can also improve the activity of antioxidant enzymes, alleviate the oxidative damage induced by lead. The detailed mechanism remains to be further explored.