| Objective:This study explored the effects of local transplantation of autologous nanofat in the treatment of rats with diabetic foot wounds.Methods:Fifty SD rats were fed with high-fat diet for four weeks,then intraperitoneally injected with streptozotocin at a dose of 35 mg/kg.Ten SD rats were fed with normal diet.Fasting blood sugar,plasma insulin concentration and glucose infusion rate were measured to determine the successful establishment of diabetic rat model.nanofat was prepared for transplantation into the ulcer area of left rear foot.Phosphate-buffered saline injection in the right rear foot served as the control treatment.We measured wound size,the extent of epithelization,the density of vessels and the expression levels of cytokines at 6 different time points post-operation.Results:The fasting blood sugar of rats fed with high fat diet was significantly higher than that of rats fed with normal diet,while the glucose infusion rate and serum insulin were significantly lower than those fed with normal diet.The diabetic wound model of rats was successfully established.nanofat-treated feet had significantly smaller wound areas than control feet at 7 and 9 days after grafting.Compared with the control feet,the nanofat-treated feet showed better re-epithelialization,a greater number of microvessels and higher levels of angiogenic factor expression.Surprisingly,mature adipocytes were found in nanofat-treated feet while viable adipocytes can't be seen after nanofat grafting in human.Further detection suggested that the different contents of Extracellular matrix(ECM)in nanofat between human and rat may attribute to this finding.Conclusion:This research shows that autologous nanofat transplantation can promote diabetic foot wound healing in rats and that it may be suitable for the clinical treatment of diabetic foot ulcers. |
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