The Analysis Of Intestinal Microflora Changes In Children With Henoch-Sch(?)nlein Purpura

Henoch-Schonlein Purpura is a common vascular inflammatory disease in children.Currently,according to the main involved parts of the lesions,HSP can be divided into 5 types:skin type(simple purpura type),abdomen type,joint type,kidney type and mixed type.with the increasing incidence of HSP,need more attention and diagnosis as soon as possible.However,the etiology and pathogenesis of HSP is still unclear,which is currently believed to be related to immune abnormalities.Previous studies prove that HSP is a kind of the IgA mediated disorder of immune reaction of antigen,the IgA by combining and activate complement factor,cross reaction with endothelial cells,damaged cells,perivascular inflammatory cell infiltration,chemokines and cytokines generated form vascular inflammation,cause humoral immunity and cellular immune disorders,mainly for Th1/Th2 imbalance,and intestinal mucosal epithelial cell damage,biological barrier function is impaired,the intestinal micro ecological destruction,intestinal flora disorder,further aggravating the immune imbalance.The structural diversity and functional stability of human intestinal flora are of great significance to human health.With the development of research,it has been found to be related to a variety of diseases,and it has been found that intestinal microflora is an important factor regulating the immune system response,and when it is out of balance,it will lead to some autoimmune and immune-mediated diseases.In recent years,studies on the immune mechanism of HSP have found that changes in intestinal flora may affect the development and outcome of HSP,mainly reflected in the decrease in the number of obligate anaerobic bacteria such as bifidobacteria and lactobacillus,and the decrease in the ratio of bifidobacteria to e.coli,which cannot fully represent the changes in intestinal flora structure.In addition,denaturation gradient gel electrophoresis,real-time fluorescence quantitative polymerase chain reaction and other traditional molecular biology technologies are mostly applied.These technologies have their limitations and cannot fully reflect the structural information of the whole community,which is time-consuming and requires a lot of work.The 16S rDNA gene is a gene encoding the 16S subunit of the ribosome of prokaryotes.Its variable region V3-4 region is often used as a marker for the classification of human intestinal flora.It can sequence millions of DNA molecules at the same timeObjective:To explore the changes of intestinal flora in children with allergic purpura(HSP)during the acute phase and convalescent phase,as well as the difference in intestinal microbiota between abdominal type and non-abdominal type allergic purpura,so as to provide reference for further understanding the pathogenesis of allergic purpura and finding new therapeutic targetsMethods:28 cases of children with HSP admitted to Jinhua Hospital of Zhejiang University from October 2016 to May 2018 were selected,including 19 cases of acute fecal specimens of children with abdominal allergic purpura,9 cases of convalescent fecal specimens,9 cases of non-abdominal HSP fecal specimens,a total of 37 cases of fecal specimens.Illumina MiSeq platform was used to extract DNA samples for high-throughput sequencing,and biological information of the sequencing results was analyzed.Results:(1)general information:among 28 hospitalized children with allergic purpura,17 were male and 11 were female,the ratio of male to female was 1.54:1.The minimum age of admission was 3 years old,the maximum age was 13 years old,and the average age was 7.35 years old.(2)the abundance of clostridium XIVa and maura in feces of acute purpura was lower than that in convalescence(Wilcox test p<0.05).(3)The relative abundance of non-ventral anaphylactic purpura in rumencoccus was higher than that of ventral type,while the relative abundance of veillonella was lower(Wilcox test,p<0.05).(4)the relative abundance of rumen coccus was the highest in the fecal specimens of the convalescence period of abdominal allergic purpura(wilcox test,p<0.05).Conclusions:The change of intestinal flora may be related to the incidence of abdominal allergic purpura.The intestinal microbiota involved in the formation of abdominal and non-abdominal allergic purpura were different.