Analysis Of The Relationship Between EB Virus Nucleic Acid And Its Encoded MicroRNAs And The Occurrence Of Henoch-Schonlein Purpura In Children

Objective:To investigate the infection of EB virus in children with Henoch-Schonlein purpura(HSP),analyze the expression of EB virus encoded microRNAs in children with HSP,and further understand the relationship between EB virus nucleic acid and its encoded microRNAs and the incidence of HSP.Method:?Retrospective investigation method was adopted.212 cases of children with HSPdiagnosed in the Children's Hospital Affiliated to Suzhou University September 2011 to January 2017 were selected as the research objects.111 cases of children with nephrotic syndrome and 208 cases of children with pneumonia were selected as the disease control group.The clinical data of the three groups and the results of EB virus DNA measurement were statistically analyzed,and the differences in the detection rate of EB virus DNA positive and viral load expression in the whole blood of the three groups were analyzed.All the data were processed by statistical methods,so as to explore the relationship between EB virus infection and HSP in children.?The method of disease control was adopted.Children with HSP diagnosed in our hospital were selected as the research objects.Children with pneumonia were taken as the disease control group,and healthy children who had physical examination in our hospital during the same period were taken as the healthy control group.EB viral DNA loads in the whole blood of the three groups were determined by real-time fluorescence quantitative PCR.EB virus DNA positive patients were selected and real-time fluorescence quantitative PCR method was used to detect the expression of EBV-encoded microRNAs in whole blood.Serological detection of EBV antigen-specific antibodies in children with HSP to understand the status of infection(latent infection and EBV reactivation).Results:1.The positive detection rate of EB virus and viral nucleic acid detection.? The positive detection rate of EBV in the three groups,HSP group(52.83%),nephrotic syndrome group(36.04%),and pneumonia group(24.04%),with statistically significant differences among groups(P<0.05).Compared with the other two groups,the positive rate of blood EBV DNA expression in HSP group was the highest.?The positive detection rate of mals and females in the three groups were HSP group(1.33:1),nephrotic syndrome group(2.47:1),and pneumonia group(1.48:1),The incidence of EBV in males was generlly higher than that in females,and there was no statistically significant difference in the positive detection rate of EBV in different genders in each research group(P>0.05).?The age group with the highest incidence was HSP group(school age,7-10 years)(42.92%),and the positive rate of EBV DNA was also the highest(60.44%).The nephrotic syndrome group was 3-6 years old(pre-school age)(43.24%),and the positive detection rate was the highest(39.58%).The pneumonia group was 3-6 years old(pre-school age)(45.67%),and the positive detection rate was the highest(27.37%).The positive detection rates of 3-6 years old and 7?10 years old in different disease groups were statistically significant(P<0.05),?2was 8.633 and 18.213,respectively.There was no significant difference in positive detection rate among other age groups(P>0.05).?The expression of EBV DNA load in pneumonia group was higher than that in HSP group(P<0.001),and higher than that in nephrotic syndrome group(P<0.001).There was no significant difference in the expression of EBV DNA load between the HSP group and the nephrotic syndrome group(P=0.297).In the group of HSP and nephrotic syndrome,the proportion of EBV DNA load in the range of 1.0 ×102?1.0×103copies/ml was the largest.The largest proportion of pneumonia group of viral load in the 1.0 ×103?1.0×104copies/ml interval,the largest proportion of all research different loads distribution interval difference was statistically significant.2.Detection of EB virus encoded microRNAs.In this part,a total of 93 cases of HSP children with positive EB virus DNA expression,78 cases of children with pneumonia and 30 cases of healthy carriers were obtained.Analyze the positive cases:?the research groups EB virus DNA load distribution range of 1.0×102?1.0×105copies/mL.The viral load of each group was mainly low copy number and there was no significant difference in the distribution of viral load among each group(P>0.05).?Detection results of EB virus antigen-specific antibody in serum showed that 75 cases(80.65%)were latent infection in the HSP group,and 18 cases(19.35%)were reactivated by EBV.In the pneumonia group,58 cases(64.10%)had latent infection,and 20 cases(35.90%)had EBV reactivation.?the expression levels of EBV-encoded microRNAs in the whole blood of HSP group were significantly higher than those of healthy carriers(P<0.05),among which the expression levels of miR-BART3-3p were the highest.The expression levels of miR-BART3-3p,1-5p,10-5p,12,13-3p and 16-1 in the HSP group were significantly higher than those in the pneumonia group(P=0.04,=0.029,=0.016,=0.001,=0.041,<0.001,respectively).miR-BART5-3p and 6-5p were detected in the HSP group,but not in the pneumonia group and healthy carriers.?Under different infection conditions,the expression levels of miR-BART1-5p and 13-3p in EBV-reactivated whole blood EBV-miRNAs in the HSP group were significantly higher than those in the children with latent infection(P=0.045,=0.032,respectively),and the expression levels of miR-BART10-5p and 16-1 in the children with pneumonia group were significantly higher than those in the children with latent infection(P=0.023,=0.042,respectively).There was no significant difference in the expression level of other detectable miRNAs between the two groups in children with latent infection and EBV reactivation(P>0.05).?Under the condition of latent infection,the expression levels of miR-BART7-3p and 13-3p in whole blood ebv-mirnas in the HSP group were significantly higher than those in the pneumonia group(P=0.006,=0.008,respectively),and were also significantly higher than those in healthy carriers(p=0.021,=0.001,respectively).But only the expression of miR-BHRF1-1,1-2-3pandmiR-BART3-3p,16-1 and miR-BART 8-5p,9-3p,11-3p,12,19-3p in the pneumonia group was higher than that in healthy carriers(P<0.001,<0.001,=0.035,=0.001,=0.020,=0.001,=0.003,=0.001,=0.005,respectively).Conclusion:1.EBV infection is closely related to the occurrence of HSP,especially the immune mechanism involved in latent infection of EBV may a important factor to induce the occurrence of HSP.2.EBV-encodedmiR-BART13-3p,7-3p,8-5p,3-5p,11-3p,19-3p,5-3p,6-5pandmiR-BHR F1-1,1-2-3p,1-2-5p may be related to the occurrence of HSP;the differential expression miR-BART13-3p,7-3p,and miR-BART5-3p and 6-5p,which are exclusively expressed in HSP,are of particular interest and research.