| Background and Purpose:Endometriosis(EMs)is a kind of disease that seriously affects the quality of life and fertility of women of childbearing age.Its clinical incidence has been increasing year by year.At present,the exact etiology and pathogenesis of endometriosis are still unclear,causing difficulties in "early diagnosis and early treatment".Recent studies have shown that brain-derived neurotrophic factor(BDNF)binds to its high-affinity receptor tyrosine kinase receptor B(TrkB)and is involved in the development of endometriosis through signaling pathways.In this study,the expression of BDNF in the serum of patients with endometriosis and its correlation with clinical characteristics were elaborated to explore its role in the occurrence and development of endometriosis and its value in clinical diagnosis.MethodsFrom June 2017 to June 2018,a total of 82 cases of endometriosis treated by gynecological surgery in ZhongShan Hospital Affiliated to XiaMen University were selected,all of which were confirmed by postoperative pathological results.In the same period,51 cases of ovarian benign tumor patients and 75 cases of healthy women of reproductive age were selected as the control group Enzyme-linked immunosorbent assay(ELISA)was performed to detect the concentration of serum BDNF levels,analyzing its correlation with endometriosis stage,dysmenorrhea and infertility.The diagnostic performance of BDNF,BDNF and CA125 combined detection for endometriosis was analyzed by using the subject operating characteristic curve.Result:The serum BDNF expression level of endometriosis patients was(1082±43.01)pg/ml,which was significantly higher than that of healthy women of childbearing age(648.80±29.53)pg/ml,there were significant statistical differences between them(t=5.269,P<0.001).And it was also statistically significant compared with that of benign ovarian tumor patients[(748.60±40.57)pg/ml,t=5.269,P<0.001].The expression level of serum BDNF in patients with r-AFS stage ?-?(1163.79±388.85)pg/ml was significantly higher than that in patients with r-AFS stage ?-?(791±218.12)pg/ml,and the difference was statistically significant(t=3.887,P<0.001).The patients without dysmenorrhea and mild dysmenorrhea in endometriosis were classified as one group,while the patients with moderate and severe dysmenorrhea were classified as one group.The difference in the expression level of BDNF between the two groups was statistically significant[(717.60±36.15)pg/ml VS(1292±42.86)pg/ml,t=9.143,P<0.001],and BDNF expression was positively correlated with dysmenorrhea(r=0.682),but there was no correlation between BDNF expression and sterility(r=0.05).According to the analysis of the subject's working characteristic curve,when the positive reference value of serum BDNF was set as 982.94 pg/ml,the sensitivity and specificity of the diagnosis of endometriosis were 62.2%and 92.0%.Respectively,while the combined detection with CA125 could increase the sensitivity and specificity of the diagnosis of endometriosis to 93.9%and 98.7%Conclusion:BDNF is highly expressed in the serum of patients with endometriosis,which is correlated with the stages of endometriosis and dysmenorrhea.The recommended positive threshold of BDNF is 982.94pg/ml,and the combined detection of BDNF and CA125 can significantly improve the diagnostic performance of endometriosis. |
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