The Protective Effects Of Brain-derived Neurotrophic Factor In Acute Mentally Stressed Rats And The Intervention Of Fluoxetine

PART I : The Effects of Acute Mental Stress on Expressionof BDNF and CREB in Some Selected Brain Regionsof Sprague-Dawley RatsObjectives: To study the dynamic changes of brain-derived neurotrophic factor (BDNF) and cAMP response element binding protein(CREB) gene expression after acute mental stress in some brain regions of rats, hoping to explore the protective effects and mechanisms of BDNF in mental stress. Methods: We applied the Communication Box to establish animal model of mental stress, then assessed their anxiety behavior through the Open field test and elevated plus maze test, and lastly, using in situ hybridization and immunohistochemistry methods, measured the expressions of both BDNF and CREB in their CA1,CA3, dentate gyrus (DG) of hippocampus, prefrontal cortex(PF), central amygdaloid nuclei(AG), shell of accumbens nucleus(NAC), periaqued-uctal gray(PAG), ventral tegmental area(VTA) and Dorsomedial hypothalamic nucleus( DM). Results: 1. The Open field tests showed that,...