| Rationales: Quality of Chinese medicinal materials and its height is closely related to the active ingredient content in the body,and the base of the original plant secondary metabolites accumulation degree and the content,in the process of planting to harvest processing affected by many factors together,and the most used to visually identify medicinal materials quality is appearance,through to summarize the appearance of the medicinal materials to form the product specifications and grades,but as a result of the original plants and yinpian processing is a more complex link process,product specifications and grades and quality of Polygala tenuifolia Willd.correlation between is not clear,at the same time,in view of the current research of P.tenuifolia efficacy material base and its chemical composition is difficult to obtain.It is difficult to find a pathological/biological model with high fit,so the correlation between the quality control index components and the clinical efficacy of medicinal materials is poor.It is urgent to establish the quality standard of P.tenuifolia with the combination of appearance and internal chemical composition,which is oriented by clinical efficacy,to regulate the planting,processing and circulation of medicinal materials and decoction pieces.In view of this,this study first adopted statistical analysis method and combined with market research to revise the commodity specification and grade standard of P.tenuifolia in the 1984 edition of commodity specification and grade standard of 76 Chinese medicinal materials,and then analyzed the change rule of chemical composition in P.tenuifolia.At the same time,human disease target enzymes PTP1 B were used to screen the activity of different polar parts of P.tenuifolia.In order to provide the basis for the improvement and revision of the standards of specifications and quality of P.tenuifolia,and to lay the foundation for ensuring the quality uniformity of P.tenuifolia and the accuracy of clinical medication.Objective: 1.To revise the standards of commodity specifications and grades of P.tenuifolia to standardize the circulation of P.tenuifolia in the market.2.The regularity of chemical composition change in P.tenuifolia was analyzed,and reasonable indexes were selected to control the quality of P.tenuifolia,which laid a foundation for the improvement and revision of commodity specification and grade standard and quality standard of P.tenuifolia.3.Based on the test data of a large number of samples,reasonable Suggestions were put forward for the revision of quality standards of radix P.tenuifolia and decoction pieces,so as to better control the quality of P.tenuifolia and decoction pieces and lay a foundation for the safety and effectiveness of clinical drug use of P.tenuifolia.4.Human disease target enzyme PTP1 B was used to screen the anti-type 2 diabetes activity of different polar parts of P.tenuifolia,laying a foundation for elucidating that P.tenuifolia may play an active role in inhibiting PTP1 B activity and thus play an effective component in anti-type 2 diabetesMethods: 1.SPSS was used to conduct statistical analysis on the appearance and character data of P.tenuifolia medicinal materials with different specifications and grades collected in the market,and the commodity specification and grade standards of P.tenuifolia medicinal materials were revised based on the current market circulation status.2.Using HPLC method(content determination/fingerprint),UPLC method(multi indexes including measurement)analysis of different product specifications and grades P.tenuifolia medicinal materials,different organizations(root/barrel/wood heart),slices of different manufacturer production(P.tenuifolia/processed drugs)for chemical variance analysis,use the statistics software such as SPSS,SIMCA analysis P.tenuifolia chemicals in the body,the change rule of P.tenuifolia medicinal materials in the 2015 edition of China pharmacopoeia and yinpian quality standards revision opinions,and USES the factor analysis based on appearance of P.tenuifolia quality evaluation system.3.The target enzyme PTP1 B of human diseases was purified by ion exchange column,and the enzyme activity inhibition system of PTP1 B was established.The activities of different polar parts of P.tenuifolia prepared by system solvent extraction method were screened.Results: 1.The current standard of commodity specification grade of was revised to make the standard more suitable for the status quo of P.tenuifoli in market circulation and play a normative role in the circulation of medicinal materials.2.The content of Polygalaxanthone III(PXIII)fluctuated greatly in different regions,but it was not closely related to the specification and grade.3,6'-disinapoyl sucrose(DISS)can be used as the index component to classify Tong and Gun,but there is no significant difference between different grades.The content of Tenuifolin in P.tenuifolia was stable compared with the other two components in each batch.The chemical compositions of P.tenuifolia from different producing areas and different specifications and grades have high similarity.The diameter had a great influence on the similarity of fingerprint in Datong,and the heart rate had a great influence on the similarity of fingerprint in Zhongtong.It was difficult for fingerprint to distinguish P.tenuifolia with different specifications and grades.The content of sucrose except DISS in 8 indicative components of P.tenuifolia from the same origin was significantly different,and the content of wild product was higher than that of cultivated product.3.The composition in the heart of P.tenuifolia is similar to that of fresh root and barrel,but the overall content is much lower than that of root and barrel.The similarity of root and tube fingerprints before and after heart pumping of a batch of medicinal materials was high,DISS can be used as an indicator to distinguish whether P.tenuifolia has heart pumping or not.It was found barrel and fresh root could be distinguished by principal component analysis with 8 index components.There was no significant difference in single component between root and barrel.4.There was no significant difference in the contents of the three indexes in the pharmacopoeia of the same batch of P.tenuifolia after processing.The quality uniformity of the slices of P.tenuifolia from different manufacturers is poor,and the fluctuation range of each component is wide.The fingerprint similarity of the same batch of medicinal materials before and after processing is high.The effect of producing area on the chemical composition of Polygalae Radix decoction pieces is greater than that of processing.After processing,the contents of Sibiricose A5,A6,Sibiricoxanthone B and Tenuifoliside A were slightly increased as A whole,but there was no significant difference between P.tenuifolia and P.tenuifolia after processing.5.After correlation analysis,the content of DISS in pharmacopoeia was significantly correlated with diameter and length,and with heart rate,the content of Tenuifolin in P.tenuifolia was significantly correlated with diameter,and the content of PXIII was not significantly correlated with diameter,length,and heart rate.Two mouth mountain and the diameter of ketone compounds(Sibiricoxanthone B,PXIII)were significantly negative correlation,and sugar esters composition Glomeratose A and Tenuifoliside A significant negative correlation,and DISS appear very significant positive correlation;The length of Sibiricose A5,A6 and DISS was significantly positively correlated with the sugar and ester components.Heart rate and the outlet is mountain ketone components Sibiricoxanthone B,has negative correlation with sugar esters composition Glomeratose A and Tenuifoliside A significant negative correlation,and DISS was significantly positively related.6.The target enzymes PTP1 B were used to screen the activity of different polar parts of P.tenuifolia,and it was found that the chloroform extract part of P.tenuifolia prepared by system solvent extraction method had better inhibitory activity against PTP1 B,suggesting that this part may have potential anti-type 2 diabetes activity.7.Macroporous resin method was successfully used to divide P.tenuifolia into two parts along the 18 min of sugar and ester mixing zone in UPLC fingerprint.The identification results showed that the majority of the components in 20% ethanol flow were sugar and ester components,and a small part of the components were xanthones.The components in 35% ethanol flow are mostly sugar esters and a small part of saponins.Conclusion: Based on the investigation to the market and the origin of this research results combined with SPSS statistical analysis method for current P.tenuifolia medicinal materials,product specifications and grades to revise,and use HPLC method(content determination/fingerprint),UPLC method(multi indexes including measurement)analysis of different product specifications and grades P.tenuifolia medicinal materials,different organizations(root/barrel/wood heart),slices of different manufacturer production(P.tenuifolia/processed drugs),the chemical differences of points put forward in the 2015 edition of China pharmacopoeia P.tenuifolia and slices quality standards revision opinions,and establish evaluation system based on the appearance of P.tenuifolia quality standards.Through in vitro PTP1 B enzyme activity inhibition screening model,the chloroform extract part of P.tenuifolia may have potential anti-type 2 diabetes activity.On the one hand,it lays a foundation for screening the effective components that inhibit PTP1 B in P.tenuifolia,and on the other hand,it provides a basis for the revision of commodity specifications,grade standards and quality standards of P.tenuifolia in subsequent,so as to ensure the quality consistency of P.tenuifolia and the effectiveness of clinical medication. |
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