Clinical Significance Of High Mobility Group Protein B1 And Mitochondrial DNA In Late-onset Sepsis In Term Infants

Objectives To detect the changes of High Mobility Group Box 1 and mitochondrial DNA damage markers levels in children with late-onset septicemia,and to explore whether there is a correlation between HMGB1 and mitochondrial DNA damage repair.Clinical significance in septal sepsis.Methods From April 2018 to February 2019,the neonatal department of Hunan Children's Hospital received 59 full-term neonates(More than 37 weeks of gestational age)aged 3-28 days.According to the infection,the subjects were divided into control group(non infection group)and common infection group and the sepsis group,blood samples were taken before admission to the hospital without antibiotics,7 days after treatment,and 14 days after treatment.The levels of plasma HMGB1 and8-OHDG were determined by ELISA.The mitochondrial HMGB1 was determined by Western Blot.?H2AX level,while routinely recording the child's gender,gestational age,age,birth weight,admission weight,Apgar score,amniotic fluid,placenta,umbilical cord;blood routine,CRP,PCT,ESR,blood culture,liver and kidney function,Laboratory indicators such as myocardial enzymes and coagulation function.The changes of HMGB1,8-OHDG and ?H2AX in different periods were observed and correlation analysis was performed.Statistical analysis was performed using SPSS 20.0.Results1.The levels of plasma HMGB1 and 8-OHDG in the sepsis group were higher than those in the normal infection group,which was higher than that in the control group,and the difference was statistically significant(P<0.05).The levels of plasma HMGB1 and 8-OHDG in the normal infection group were lower than those at admission.The plasma levels of HMGB1 and 8-OHDG in the normal sepsis group and the septic organ function impairment group were higher than those at admission,7 days after admission.The difference was statistically significant(P < 0.05).2.Compared with the control group and the general infection group,the ratio of HMGB1 to ?-actin in the mitochondria was lower,the expression was significantly decreased,and the expression of HMGB1 gradually increased with the improvement of the condition.The expression of?H2AX and ?-actin in sepsis was higher,and the expression was significantly increased.The expression of ?H2AX decreased gradually with the improvement of the disease(P<0.05).3.Correlation analysis of plasma HMGB1 and 8-OHDG was r=0.741,P<0.05.The higher the level of HMGB1 in plasma,the higher the8-OHDG level was positively correlated.The correlation between HMGB1 and ?H2AX in mitochondria was r=-0.302,P<0.05,which was correlated,but the correlation was not significant.Conclusions1.The levels of plasma HMGB1 and 8-OHDG were significantly increased in full-term septicemia in term infants;HMGB1 levels in mitochondria were significantly decreased,and ? H2 AX levels were significantly increased,which was associated with mt DNA damage and repair.2.There was a positive correlation between the changes of plasma HMGB1 and 8-OHDG levels.