Expression And Significance Of TREM2 In Peripheral Blood Of Patients With Hashimoto's Thyroiditis

Objective: Hashimoto's thyroiditis(HT)is one of the Autoimmune Thyroid Diseases(AITD).With the development of thyroid disease,the final outcome of most HT is the loss of thyroid secretory hormone function.The main cause of HT is autoimmune factors,and many basic and clinical studies have confirmed that multiple types of cytokines act simultaneously on the development of HT disease.The main subject of this study was the first diagnosis of Hashimoto's thyroiditis.The proportion of Triggering receptor expressed on myeloid cells 2(TREM2)in peripheral blood mononuclear cells(Peripheral blood mononuclear cells,PBMC)was explored.The significance of TREM2 in the progression of HT disease provides a new research direction for the theoretical system of HT disease progression mechanism.Methods: The study was divided into experimental group and control group.Experimental group: between 2018.01.01 ~ 2018.12.31,the patient in the endocrinology clinic of the Affiliated Hospital of Jining Medical College,the first diagnosis of HT and the eligible patients,a total of 60 patients,including 2 males and 58 females.The control group: 40 healthy people in the physical examination center of the Affiliated Hospital of Jining Medical College,including 3 males and 37 females,whose age,gender and experimental group were not significantly different,and did not have autoimmune Disease,no history of infectious diseases within six months.The HT group and the HC group were selected strictly according to the inclusion and exclusion criteria.At 8o'clock in the morning under fasting conditions,4 ml of peripheral venous blood of the experimental group and the control group were taken and stored in 2 tubes,2 ml per tube,and used.One of the tubes was taken and the concentrations of FT3,FT4,TSH,TPOAb and TgAb were determined by chemiluminescence immunoassay.Another tube of venous blood was centrifuged at 1000 r/min by a centrifuge to separate the upper serum and the lower blood cells.The supernatant was aspirated,and the expression levels of IL-17 and IFN-? were measured by enzyme-linked immunosorbent assay(ELISA).Lower layer sedimentation was measured by flow cytometry in the level of TREM2 in peripheral blood PBMC.Results: The proportion of TREM2 in peripheral blood of HT group was significantly lower than that of HC group(P<0.01),and the levels of IFN-?,IL-17,TPOAb and TgAb were significantly higher than those of HC group(P<0.01,P<0.01,P<0.01,P<0.01,P<0.01).The results of bivariate correlation analysis showed that the level of TREM2 in peripheral blood of HT patients was significantly negatively correlated with serum IL-17,IFN-?,TPOAb and TgAb(r=-0.315,P<0.01;r=-0.259,P= 0.017;r =-0.232,P= 0.021;r =-0.219,P = 0.028).Conclusion: The amount of TREM2 in PBMC of HT patients was significantly lower than that in HC group,and the proportions of IFN-?,IL-17,TPOAb and TgAb were significantly higher than those in healthy controls,and TREM2 levels and IFN-? in HT patients.TPOAb and TgAb showed strong negative correlation,suggesting that TREM2 may interfere with the progression of HT by affecting the secretion of IFN-?,IL-17 and other factors.This study provides a new direction for us to explore the theoretical system of disease progression in HT,providing clinicians with different treatment directions and sites.