| Objective 1.Studying the distribution of TCM syndromes in chronic obstructive pulmonary disease patients of grade Ⅰ and Ⅱ.2.Studying the effect of SQHTF on the expression of inflammatory markers in COPD rats.Methods 1.Clinical research: Formulate a questionnaire of TCM syndromes to collect the general situation and four diagnostic data of chronic obstructive pulmonary disease patients of grade Ⅰ and Ⅱ,then summarize the distribution of TCM syndromes by data analysis.2.Animal experiment: SD male rats were divided into control group and model group according to random number table.COPD rat model was established by intratracheal LPS infusion combined with smoking.After successful modeling,the rats were further randomly divided into model group,treatment group and JSB group.After 4 weeks of drug intervention,the lung function of rats in each group was measured,the morphological changes of lung tissue were observed,the levels of serum IL-6 and IL-1beta were detected by ELISA,and the expressions of c-fos and c-jun protein in lung tissue were detected by Western Blot method.Results 1.Clinical research: The most common syndromes of COPD patients of grade Ⅰ and Ⅱ are phlegm turbidity obstructing lung and lung-qi deficiency(19.42%),phlegm turbidity obstructing lung and lung-kidney Qi deficiency(15.53%),phlegm-heat obstructing lung and lung-qi deficiency(12.62%),wind-cold attacking lung and lung-qi deficiency(11.65%),phlegm turbidity obstructing lung,lung-qi deficiency and blood stasis(6.80%)and lung-qi deficiency(6.80%).2.Animal experiments 2.1 Pulmonary function index Compared with the control group,the pulmonary function indexes of each group decreased in different degrees,with statistical significance(P < 0.05);compared with the model group,the pulmonary function indexes of each group improved after treatment,with statistical significance(P< 0.05);compared with Jinshuibao group,the FEV0.3 and FEV0.3/FVC indexes of the treatment group improved more significantly,with statistical significance(P< 0.05).2.2 Histomorphology of Lung In the control group,the lumen was unobstructed,the alveolar structure was intact,and the lung tissue structure was basically normal.In model group,the small airway was obviously narrowed,alveolar septum was broken,alveolar cavity was fused,lymphocyte infiltration was obvious,which accorded with the pathological changes of COPD.Compared with the model group,the tissue damage of rats in each group was alleviated after drug intervention,especially in the treatment group.2.3 Expression of IL-6 and IL-1beta Compared with the control group,the expression of IL-6 and IL-1beta in serum of rats in each group increased significantly(P < 0.01).Compared with the model group,the expression of IL-6 and IL-1beta in serum of rats in the two groups decreased significantly after drug intervention(P < 0.01).Compared with Jinshuibao group,the expression of IL-6 in the treatment group decreased significantly(P < 0.05),but the expression of IL-1beta in the two groups was no statistical difference.2.4 Expression of c-fos and c-jun Compared with the control group,the expression of c-fos and c-jun in lung tissue of rats in each group increased significantly(P < 0.01).Compared with the model group,the expression of c-fos and c-jun in lung tissue of rats in the two groups decreased significantly after drug intervention(P < 0.01).Compared with Jinshuibao group,the expression of c-fos and c-jun in the treatment group decreased significantly(P < 0.05).conclusion 1.Most of the TCM syndromes of COPD patients of grade Ⅰ and Ⅱ are are combined with deficiency and excess;mild COPD patients mainly have deficiency of lung-qi,while moderate COPD patients involve spleen and kidney.The development of COPD will lead to the increase of the involved viscera.2.SQHTF can regulate the expression of IL-6 and IL-1beta by inhibiting the transcription factor AP-1,alleviate airway inflammation and reduce lung tissue injury. |
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