The Neuroprotective Effect Of LX11 On SH-SY5Y Neurons Induced By Oxygen-glucose Deprivation/Resupply Injury

Background:Ischemic stroke ranks first among stroke patients in China,accounting for about 78%.At present,thrombolytic therapy is the most effective therapy.Thrombolytic therapy can restore the blood flow supply in ischemic brain tissue,and bring great clinical benefit to patients.At the same time,cerebral ischemia-reperfusion(I/R)injury can also be caused by reperfusion blood flow in ischemic area.Apocynin is a natural plant polyphenol compound,namely methoxy-catechol,which is a specific inhibitor of NADPH oxidase.LX11 is a new plant polyphenol compound synthesized with apocynin as a lead compound.Objective:In this study,we used human neuroblastoma cells(SH-SY5 Y neurons)cultured in vitro to establish and screen a successful cell model of oxygen-glucose deprivation-resupply(OGD/R)injury to simulate I/R injury in vivo.On this basis,it is further clear whether LX11 has protective effect on the injury model.It provides a reliable experimental basis for the next step to study the neuroprotective effect and molecular mechanism of LX11 in vivo and in vitro.Methods:The frozen SH-SY5 Y neurons were resuscitated and subcultured,a successful oxygen-glucose deprivation model was established using 2,2-azobis(2-methylenedimide)dihydrochloride(AAPH)-glucose free DMEM solution.CCK-8 assay was used to detect the concentration-effect relationship and time-effect relationship of LX11 in protecting the injury model,so as to determine the appropriate concentration and time for the neuroprotective effect of the drug on the injury model.TUNEL assay was used to detect the anti-apoptosis effect of LX11 at the appropriate concentration and time of action on SH-SY5 Y neurons with oxygen-glucose deprivation/resupply injury.Results:1.The SH-SY5 Y neurons were successfully resuscitated,subcultured,and cryopreserved,the AAPH solution with concentration of 40 mmol for 3 h was successfully selected as the modeling condition of SH-SY5 Y neuron oxygen glucose deprivation / resupply model.2.LX11 had a better protective effect on SH-SY5 Y neurons induced by glucose-oxygen deprivation/resupply injury at a concentration of 200?mol for 48 hours.3.LX11 with a concentration of 200?mol significantly reduced apoptosis of injury model for 48 hours.Conclusions:LX11 has a protective effect on neurons after oxygen-glucose deprivation/resupply injury.