Mechanism Of Estrogen Inducing Angiogenesis In Lung Adenocarcinoma By Regulating VEGF? Expression

Objective:To investigate the mechanism of 17?-estradiol induced tumor angiogenesis by VEGF?in the microenvironment of lung adenocarcinoma in A549 cells.Methods:A549 cells were divided into three groups:PBS group,10nmol·L^-117?-estradiol?E2?group and 10nmol·L^-1E2+5?mol·L^-1estrogen receptor antagonist?ICI?group.Western blot?WB?was used to detect the expression of VEGF?in A549 cells,ELISA was used to detect the expression of VEGF?in culture medium.The proliferation of human umbilical vein endothelial cell?HUVEC?was detected by MTT,the migration ability of HUVEC by scratch assay and the tubulogenesis in vitro by tube formation assay.Results:Compared with the control group,the expression of VEGF?in A549 cells in17?-estradiol?E2?group was higher,but the expression of VEGF?in A549 cells was significantlyinhibitedwiththeadditionofestrogenreceptorantagonist?ICI?.Meanwhile,E2 induced the expression of VEGF?in A549 cells,promoting proliferation,migration and tubulogenesis of HUVEC,while ICI has the opposite effects.Conclusions:17?-Estradiol stimulates the expression of VEGF?by binding to estrogen receptor in A549 cells,promoting proliferation,migration and tube formation of HUVEC,contributing to lung adenocarcinoma angiogenesis.